【摘要】 为明确湖南某规模奶牛场临床型乳房炎病原菌种类及耐药情况，对32份临床型乳房炎患牛乳样进行传统细菌培养、生化试验、16S rDNA核苷酸序列检测和药敏试验。结果共分离鉴定大肠杆菌23株，乳房链球菌12株，肺炎克雷伯氏菌5株，样品检出率分别为71.9%(23/32)、37.5%(12/32)、15.6%(5/32)；药敏试验显示，大部分大肠杆菌和乳房链球菌对青霉素、阿莫西林、头孢他啶、庆大霉素、林可霉素耐药严重，耐药率达50%～86.9%，对亚胺培南、四环素及红霉素较敏感，敏感率达75%～82.6%；肺炎克雷伯氏菌对大部分抗生素耐药程度较低，对亚胺培南、阿米卡星、四环素、红霉素敏感率为100%。建议该养殖场抓好环境卫生、提高生物安全，建立科学、合理治疗方案。更多还原

【Abstract】 To ascertain the pathogenic species and drug resistance of clinical dairy mastitis in a large-scale dairy farm in Hunan, 32 clinical-type dairy mastitis samples were subjected to traditional bacterial culture, biochemical tests,16S rDNA nucleotide sequence detection and drug sensitivity tests. The results showed that a total of 23 strains of E. coli, 12 strains of Streptococcus udder and 5 strains of Klebsiella pneumoniae were isolated and identifed without duplication, and the detection rates of the samples were 71.9%(23/32), 37.5%(12/32) and 15.6%(5/32),respectively. The drug sensitivity tests showed that most of the E. coli and Streptococcus udder were resistant to penicillin, amoxicillin, ceftazidime, gentamicin and lincomycin serious, resistance rate of 50% ～ 86.9%, to imipenem, tetracycline and erythromycin more sensitive, sensitivity rate of 75%～82.6%. The Klebsiella pneumoniae to most antibiotics bacteria resistance degree is low, to imipenem, amikacin, tetracycline, erythromycin sensitivity rate of 100%. It is suggested that the farm should grasp environmental hygiene, improve biosecurity, and establish a scientifc and reasonable treatment plan.更多还原