【摘要】 采用UASB反应器,接种厌氧氨氧化菌自富集启动的PDA污泥,在温度由25℃下降到19℃条件下逐步将进水氮浓度从20mg/L提高到100mg/L(即氮负荷从3.2g/(m~3·d)提高到4.0g/(m~3·d)),反应器共运行115d,总氮去除率最高可达到89.9%.16S rRNA基因测序结果表明,Candidatus Brocadia作为主要厌氧氨氧化功能菌在生物填料中丰度达到3.7%,短程反硝化功能菌Thaurea在絮体污泥中的丰度最高达15.7%.宏基因组学分析显示与短程反硝化相关的基因Nar,Nap和与厌氧氨氧化相关的Nir,Hzs和Hdh基因表达程度较高.更多还原

【Abstract】 A UASB inoculated with PDA sludge initiated by anammox bacteria self-enrichment was used to gradually increase the influent nitrogen concentration from 20mg/L to 100mg/L at 25°C down to 19°C(the nitrogen load increased from 3.2g/(m~3·d) to 4.0g/(m~3·d)). The reactor was operated for 115 days with a maximum total nitrogen removal efficiency of 89.9%. 16S rRNA gene sequencing results showed that Candidatus Brocadia as the main anammox functional bacterium reached 3.7% abundance in the bio-carriers and the partial denitrifying functional bacterium Thaurea reached a maximum abundance of 15.7% in the floc sludge.Metagenomic analysis showed that the genes Nar and Nap, associated with partial denitrification, and Nir, Hzs and Hdh, associated with anammox, were fully expressed.更多还原