【摘要】 为明确贵州省火龙果病毒种类和复合侵染类型，以及主产区病毒发生动态。采集贵州省罗甸县、望谟县、贞丰县、镇宁县火龙果果园的火龙果茎共703份，利用CVX、CVX-NTU、PiVX、ZyVX和Sch VX特异性引物对疑似病毒病样品进行RT-PCR检测。结果表明：2021、2022年罗甸县火龙果病毒检出率分别为85.92%、94.90%，望谟县分别为93.48%、93.90%，贞丰县分别为90.99%、99.02%，镇宁县分别为94.52%、94.17%；其中，望谟县在2021年未检出Sch VX,2022年则有了较高的检出率（均为复合侵染），表明该病毒在望谟县扩散传播。2021、2022年罗甸县火龙果病毒复合侵染检出率分别为83.10%、90.82%，望谟县分别为93.48%、87.80%，贞丰县分别为90.09%、99.02%，镇宁县分别为93.15%、93.34%。从复合侵染的类型看，2种病毒复合侵染有9种类型，3种病毒复合侵染有10种类型，4种病毒复合侵染有5种类型，另有5种病毒复合侵染的类型。贵州省火龙果的病毒侵染以几种PVX属病毒复合侵染为主，且已在贵州省火龙果主产区大面积发生。更多还原

【Abstract】 To clarify the types and mixed infection types of Guizhou pitaya viruses, as well as the dynamics of virus occurrence in the main production areas. A total of 703 pitaya stems were collected from pitaya orchards in Luodian County,Wangmo County, Zhenfeng County, and Zhenning County, Guizhou Province. Suspected viral disease samples were detected by RT-PCR using CVX, CVX-NTU, Pi VX, Zy VX, and Sch VX specific primers. The detection rates of pitaya virus infection in Luodian in 2021 and 2022 were 85.92% and 94.90%, Wangmo was 93.48% and 93.90%, Zhenfeng was 90.99% and 99.02%, and Zhenning was 94.52% and 94.17%; Among which,in 2021, Sch VX was not detected in Wangmo, and in 2022,there was a high detection rate(both of which were mixed infection), indicating the spread of Sch VX in Wangmo. In 2021and 2022, the mixed infection detection rates of pitaya virus were 83.10% and 90.82% in Luodian, 93.48% and 87.80% in Wangmo, 90.09% and 99.02% in Zhenfeng, and 93.15% and 93.34% in Zhenning. From the detection perspective of mixed infection types, 9 types were detected of 2 viruses mixed infection, 10 types of 3 viruses mixed infection were detected, 5 types of 4 viruses mixed infection were detected, and the type of 5 virus mixed infection. The virus infection of Guizhou pitaya is mainly caused by a combination of several PVX viruses, and has occurred extensively in the main pitaya production areas of Guizhou.更多还原