【摘要】 为明确导致山东省某鸽场乳鸽大规模发病死亡的病原种类及其基因型,对4只病死乳鸽进行了病理剖检、细菌分离培养、相关病毒核酸检测和遗传进化分析。结果显示:病死鸽气管、肠道、腺胃等组织脏器出血严重,细菌分离培养无菌落生长,鸽新城疫病毒(Newcastle disease virus,NDV)、鸽轮状病毒(pigeon rotavirus,PiRV)和鸽圆环病毒(pigeon circovirus,PiCV)核酸阳性。从病死鸽中检测出的NDV属于基因VI.2.1.1.2.2亚型,与我国参考株SH-167同源性最高;检测出的PiRV属于G18P[17]基因型,与我国参考株CHN/ARO、SX-05同源性最高;检测出的PiCV与我国SF-80参考株同源性最高。结果表明,该鸽场乳鸽的发病死亡由NDV、PiCV和PiRV 3种病原混合感染引起,相关流行毒株未发生较大变异。结果提示,鸽群中流行病原较复杂,除应加强生物安全管理外,还应加强鸽群疫苗免疫,以降低鸽病暴发与流行风险。更多还原

【Abstract】 In order to identify the pathogens and genotypes causing mass morbidity and mortality to young pigeons in a pigeon farm in Shandong Province,pathological dissection,bacterial isolation and cultivation,virus nucleic acid detection and genetic evolutionary analysis were carried out on 4 dead young pigeons.The results showed that the trachea,intestine,glandular stomach and other tissues or organs of these pigeons were severely bleeding.No colony was observed after bacterial isolation and cultivation.Positive nucleic acids of Newcastle disease virus(NDV), pigeon rotavirus(PiRV) and pigeon circovirus(PiCV) were detected.The detected NDV belonged to subtype VI.2.1.1.2.2,and was the highest identical to the reference strain of SH-167 in China, while the detected PiRV belonged to subtype G18P[17], and was the highest identical to the reference strains of CHN/ARO and SX-05 in China;the detected PiCV was the highest identical to the reference strain of SF-80 in China.In conclusion,the pigeons died of mixed infection with NDV,PiCV and PiRV,and no major mutation was found in relevant epidemic virus strains.In view of complex pathogens prevalent in pigeon flocks, pigeon flocks should be intensively vaccinated,in addition to strict biosafety management,thus to reduce potential risk of outbreak and prevalence of pigeon diseases.更多还原