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2021—2023年湖南省无害化处理场CSFV、PRV、PRRSV污染情况调查
Investigation on the Contamination with CSFV,PRV and PRRSV in Bio-safety Disposal Plants in Hunan Province from 2021 to 2023
【摘要】 为了解湖南省2021—2023年无害化处理场猪瘟病毒(CSFV)、伪狂犬病病毒(PRV)、猪繁殖与呼吸综合征病毒(PRRSV)污染情况,采用RT-qPCR方法对采集的1 825份淋巴结、脾脏、耳尖等组织样品进行CSFV、PRV、PRRSV核酸检测,并对2022年衡南县PRRSV阳性样品进行分型检测及Nsp2基因测序。结果显示:CSFV、PRV、PRRSV的阳性检出率分别为3.01%、1.81%、30.14%;双重污染率为2.52%,以PRRSV与其他2种病原双重污染为主,三重污染率较低,为0.05%。2022年衡南县无害化处理场的PRRSV阳性检出率为24.12%,以春、冬季阳性率较高;北美型(NA-PRRSV)、高致病型(HP-PRRSV)、类NADC30毒株(NADC30-like)阳性检出率分别为98.39%、20.97%、43.55%;经Nsp2基因测序,1条序列存在“1+29”个不连续氨基酸缺失,为HP-PRRSV毒株,17条序列存在“111+1+19”个不连续氨基酸缺失,为类NADC30毒株。结果表明:2021—2023年,湖南省无害化处理场CSFV、PRV污染率维持在较低水平,PRRSV污染率较高,呈逐年上升趋势,其中类NADC30毒株为优势流行毒株,建议持续做好猪瘟及猪伪狂犬病的免疫接种及效果评估工作,加强PRRSV的遗传变异监测及养殖场的饲养管理和生物安全管理,以保障猪群生产安全。
【Abstract】 In order to investigate the contamination with classical swine fever virus(CSFV),pseudo rabies virus(PRV)and porcine reproductive and respiratory syndrome virus(PRRSV) in bio-safety disposal plants in Hunan Province from 2021 to 2023,1 825 tissue samples including lymph nodes,spleens and ear tips were detected for nucleic acids of the three viruses by RT-qPCR,and positive samples of PRRSV from Hengnan County in 2022 were typed and sequenced for Nsp2 gene.The results showed that the positive detection rates of CSFV,PRV and PRRSV were 3.01%,1.81% and 30.14%,respectively;the double infection rate was 2.52%,which was mainly contributed by PRRSV and other two pathogens,but the triple infection rate was lower,only 0.05%.The positive detection rate of PRRSV was 24.12% in bio-safety disposal plants in Hengnan County in 2022,especially in spring and winter;that of North American type(NA-PRRSV),highly pathogenic type(HP-PRRSV) and NADC30 like strain(NADC30 like) were98.39%,20.97% and 43.55%,respectively;based on Nsp2 gene sequencing, "1+29 " discontinuous amino acids were deleted in one sequence determined as HP-PRRSV strain,and "111+1+19" discontinuous amino acids were deleted in 17 sequences determined as NADC30-like strains.In conclusion,CSFV and PRV were available in bio-safety disposal plants in Hunan Province at a low contamination rate from 2021 to 2023,except PRRSV with a high and increasing contamination rate,especially NADC30-like strains that were dominant,with an increasing trend year by year.For safeguarding the safety of pig production,vaccination against classical swine fever and pseudorabies should be continuously implemented with evaluation on the immune effects,genetic variation of PRRSV should be intensively monitored, feeding and bio security on farms should be rigorously managed.
【Key words】 bio-safety disposal plant; PRRSV; PRV; CSFV; mixed infection;
- 【文献出处】 中国动物检疫 ,China Animal Health Inspection , 编辑部邮箱 ,2024年03期
- 【分类号】S858.28
- 【下载频次】23