【摘要】 目的 探究低强度脉冲超声(LIPUS)治疗早发性卵巢功能不全(POI)大鼠对其卵巢组织炎症的影响。方法 将45只SD大鼠随机分为对照组(n=15)、模型组(n=15)以及LIPUS组(n=15)。LIPUS辐照大鼠卵巢15 d后，采用RNA测序筛选治疗前后的差异基因并富集出相关信号通路；蛋白质免疫印迹检测(Western blot)与实时荧光定量聚合酶链反应(qPCR)检测各组大鼠卵巢组织中相关炎症因子的表达水平。结果 LIPUS治疗前后差异基因显著富集到了IL-17、NK-κB以及Chemokine信号通路之上；与对照组相比，模型组IL-1β、NF-κB、TNF-α、IL-6、IL-17、S00A8、S00A9、CXCL1、CXCL3等与炎症相关因子蛋白及mRNA表达水平均显著增高(P<0.05),而LIPUS组相较于模型组均显著降低(P<0.05)。结论 LIPUS能抑制卵巢组织中炎症因子的表达，调控趋化因子、IL-17与NK-κB信号通路，从而修复卵巢组织结构与功能。更多还原

【Abstract】 Objective To explore the effect of low intensity pulsed ultrasound(LIPUS) on ovarian tissue inflammation in rats with premature ovarian insufficiency(POI). Methods A total of 45 Sprague-Dawley(SD) rats were randomly divided into the control group(n=15), the model group(n=15), and the LIPUS group(n=15). After 15 days of LIPUS irradiation, the differential genes before and after treatment were screened by RNA sequencing and related signaling pathways were enriched. The expression levels of related inflammatory factors in ovarian tissues of rats were detected by Western blot and quantitative qPCR. Results Before and after LIPUS treatment, differential genes were significantly enriched on IL-17, NK-κB and chemokine signaling pathways. Compared with the control group, the protein and mRNA expression levels of inflammatory factors such as IL-1β, NF-κB, TNF-α, IL-6, IL-17, S00A8, S00A9, CXCL1 and CXCL3 in the model group were significantly increased(P<0.05). While compared with the model group, the expression levels of these factors in the LIPUS group were significantly decreased(P<0.05). Conclusions LIPUS may repair the ovarian tissue structure and function by reducing the expression of inflammatory factors in ovarian tissue and regulating Chemokine, IL-17 and NF-κB pathway.更多还原