【摘要】 目的 研究广西地不容块根化学成分及其酪氨酸酶抑制、杀虫活性。方法 广西地不容块根70%乙醇提取物采用Sephadex LH-20、MCI、ODS、半制备HPLC、HSCCC进行分离纯化，根据理化性质及波谱数据鉴定所得化合物的结构。以左旋多巴为底物，测定酪氨酸酶抑制活性。以柑橘木虱防治效果为指标，评价杀虫活性。结果 从中分离得到12个化合物，分别鉴定为延胡索乙素（1）、去氢克班宁（2）、crebanine（3）、stephanine（4）、liriodenine（5）、piperumbellactam A（6）、sinoacutine（7）、（+）-salutaridine N-oxide（8）、bisnorargemonine（9）、（+）-corytuberine（10）、sebiferine（11）、palmatrubine（12）。化合物5～7对酪氨酸酶IC₅₀值分别为（0.170 2±0.010 1）、（0.766 3±0.033 1）、（0.519 3±0.007 5）mg/mL。化合物2～5、7、8、10～12对柑橘木虱的防治效果在（19.33±0.57）%～（77.15±0.45）%之间。结论 化合物2、5、6、8～12为首次从该植物块根中分离得到，6、9为首次从千金藤属植物中分离得到。化合物5～7具有较强的酪氨酸酶抑制活性，7、8、10具有较强的杀虫活性。更多还原

【Abstract】 AIM To study the chemical constituents from the root tubers of Stephania kwangsiensis H.S.Lo and their tyrosinase inhibition and insecticidal activities.METHODS The 70% ethanol extract from root tubers of S. kwangsiensis was isolated and purified by Sephadex LH-20, MCI, ODS, semi-prepative HPLC and HSCCC, then the structures of obtained compounds were identified by physicochemical properties and spectral data. The tyrosinase inhibitory activities were determined by using levodopa as substrate, and the insecticidal activities were evaluated by the control effect of Diaphorina citri.RESULTS Twelve compounds were isolated and identified as tetrahydropalmatine（1）, dehydrocrebanine（2）, crebanine（3）, stephanine（4）, liriodenine（5）, piperumbellactam A（6）, sinoacutine（7）,（+）-salutaridine N-oxide（8）, bisnorargemonine（9）,（+）-corytuberine（10）, sebiferine（11） and palmatrubine（12）. The IC₅₀ values of compounds 5-7 to tyrosinase were（0.170 2±0.010 1）,（0.766 3±0.033 1） and（0.519 3±0.007 5） mg/mL, respectively. The control effects of compounds 2-5, 7, 8, 10-12 against D. citri ranged from（19.33±0.57）% to（77.15±0.45）%.CONCLUSION Compounds 2, 5, 6, and 8-12 are isolated from this plant for the first time, 6 and 9 are first obtained from genus Stephania. Compounds 5-7 displayed significant tyrosinase inhibition activities. Compounds 7, 8 and 10 show strong insecticidal activities.更多还原