【摘要】 目的 建立HPLC法同时测定脑脉泰胶囊中3′-羟基葛根素、2-羟基红花黄色素A、葛根素、葛根素芹菜糖苷、3′-甲氧基葛根素、大豆苷、二苯乙烯苷、木犀草苷、染料木苷、木犀草素、丹酚酸B、迷迭香酸的含量。方法 分析采用Kromasil C₁₈色谱柱（250 mm×4.6 mm, 5μm）;流动相乙腈-水（含1.0 g/L磷酸）,梯度洗脱；体积流量0.8 mL/min;柱温30℃;检测波长280 nm。再进行聚类分析、主成分分析。结果 12种成分在各自范围内线性关系良好（r>0.999 0）,平均加样回收率97.07%～98.30%,RSD 0.86%～1.82%。10批样品聚为4类，4种主成分累积方差贡献率达86.262%。结论 该方法简便稳定，可靠准确，可为脑脉泰胶囊的质量控制提供科学依据。更多还原

【Abstract】 AIM To establish an HPLC method for the simultaneous content determination of 3′-hydroxy-puerarin, hydroxysafflor yellow A, puerarin, puerarin apigenin, 3′-methoxypuerarin, daidzein, stilbene glycoside, luteolin side, genistein side, luteolin B and rosmarinic acid in Naomaitai Capsules.METHODS The analysis was performed on a 30 ℃ thermostatic Kromasil C₁₈ column（250 mm×4.6 mm, 5 μm）,with the mobile phase comprising of acetonitrile-water（containing 1.0 g/L phosphoric acid） flowing at 0.8 mL/min in a gradient elution manner, and the detection wavelength was set at 280 nm. Subsequently, principal component analysis and cluster analysis were made.RESULTS Twelve components showed good linear relationships within their own ranges（r>0.999 0）,whose average recoveries were 97.00%-99.00%with the RSDs of 0.86%-1.82%. Ten batches of samples were clustered into four categories, the accumulative variance contribution rate of four principal components reached 86.262%.CONCLUSION This simple, stable, reliable and accurate method can be used for the quality control of Naomaitai Capsules.更多还原