【摘要】 目的 对山银花茎叶化学成分进行分离和结构鉴定，并进行体外抗炎活性评价。方法 山银花茎叶80%甲醇提取物采用Diaion HP20SS、Sephadex LH-20、高速逆流色谱、反相半制备液相色谱等进行分离纯化，根据理化性质及波谱数据鉴定所得化合物的结构。通过测定单体化合物抑制LPS诱导小鼠巨噬细胞RAW264.7释放NO水平评价其抗炎活性。结果 从山银花茎叶中分离得到13个化合物，分别鉴定为苄醇-O-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖苷(1)、獐牙菜苷(2)、表断马钱子苷半缩醛内酯(3)、马钱子苷半缩醛内酯(4)、裂环马钱苷(5)、断氧化马钱苷(6)、裂马钱素二甲基乙缩醛(7)、绿原酸甲酯(8)、芹菜素-7-O-β-D-葡萄糖苷(9)、木犀草素-7-O-β-D-葡萄糖苷(10)、野漆树苷(11)、木犀草素-7-O-α-L-吡喃阿拉伯糖(1→6)-β-D-吡喃葡萄糖苷(12)、忍冬苷(13);化合物2～8、11～13可抑制LPS诱导RAW264.7细胞释放NO。结论 化合物1首次从忍冬属植物中分离得到，化合物3、5、7、9、11～12首次从山银花茎叶中发现。化合物2～8、11～13具有一定的抗炎活性。更多还原

【Abstract】 AIM To study the chemical constituents and their anti-inflammatory activities of stems and leaves of Lonicera confusa DC.METHODS The 80% methanol extract from stems and leaves of L. confusa DC was isolated and purified by Diaion HP20SS, Sephadex LH-20, HSCCC and preparative HPLC, then the structures of obtained compounds were identified by physicochemical properties and spectral data. Their anti-inflammatory activities were evaluated by measuring NO production of LPS-stimulated RAW264.7 cells in vitro.RESULTS Thirteen compounds were isolated and identified as benzyl alcohol-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(1), sweroside(2), epi-vogeloside(3), vogeloside(4), secologanoside(5), secoxyloganin(6), secologanin dimethyl acetal(7), methyl chlorogenate(8), apigenin-7-O-β-D-glucopyranoside(9), luteolin-7-O-β-D-glucopyranoside(10), rhoifolin(11), luteolin-7-O-α-L-arabinopyranosyl(1→6)-β-D-glucopyranoside(12), and lonicerin(13). Compounds 2-8, 11-13 inhibited the NO production of LPS-induced cells.CONCLUSION Compound 1 is first isolated from family Lonicera, compounds 3, 5, 7, 9, 11, and 12 are obtained from the stems and leaves of this plant for the first time. Compounds 2-8, 11-13 exhibited anti-inflammatory activities.更多还原