【摘要】 目的 建立一测多评法同时测定天麻头风灵胶囊中天麻素、巴利森苷E、紫丁香苷、巴利森苷B、巴利森苷C、阿魏酸、巴利森苷A、蒙花苷、哈巴俄苷、肉桂酸的含量。方法 分析采用GL Science Inertsil^TM ODS-3色谱柱（150 mm×4.6 mm, 5μm）;流动相乙腈-0.1%磷酸，梯度洗脱；体积流量1.0 mL/min;柱温30℃;检测波长220、280 nm。以紫丁香苷为内标，计算其他9种成分的相对校正因子，测定其含量。结果 10种成分在各自范围内线性关系良好（r≥0.999 7）,平均加样回收率98.53%～102.22%,RSD 1.26%～2.68%。一测多评法所得结果与外标法接近。结论 该方法准确、专属性强，可用于天麻头风灵胶囊的质量控制。更多还原

【Abstract】 AIM To establish a quantitative analysis of multi-components by single-marker（QAMS） method for the simultaneous content determination of gastrodin, parishin E, syringin, parishin B, parishin C, ferulic acid, parishin A, buddleoside, harpagoside and cinnamic acid in Tianma Toufengling Capsules.METHODS The analysis was performed on a 30 ℃ thermostatic GL Science Inertsil^TM ODS-3 column（150 mm×4.6 mm, 5 μm）, with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelengths were set at 220, 280 nm. Syringin was used as an internal standard to calculate the relative correction factors of the other nine constituents, after which the content determination was made.RESULTS Ten constituents showed good linear relationships within their own ranges（r≥0.999 7）, whose average recoveries were 98.53%-102.22% with the RSDs of 1.26%-2.68%. The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This accurate and specific method can be used for the quality control of Tianma Toufengling Capsules.更多还原