【摘要】 目的 研究窄孢灵芝Ganoderma angustisporum J.H. Xing, B.K. Cui&Y.C. Dai的化学成分及其α-葡萄糖苷酶抑制活性。方法 窄孢灵芝乙酸乙酯提取物采用硅胶、ODS、TLC、HPLC进行分离纯化，根据理化性质及波谱数据鉴定所得化合物的结构。采用pNPG法评价其α-葡萄糖苷酶抑制活性。结果 从中分离得到7个化合物，分别鉴定为N-acetyl-L-phenylalanine ethyl ester（1）、N-acetyl-L-phenylalanine methyl ester（2）、4-hydroxy-17R-methylincisterol（3）、6,8-di-O-methylaverufin（4）、aversin（5）、methyl 2-（4-hydroxyphenyl） acetate（6）、5-甲苯-1,3-二醇（7）。化合物1～2、4～7的α-葡萄糖苷酶抑制活性IC₅₀值分别为（33.80±0.47）、（45.45±7.95）、（48.80±5.86）、（39.48±2.82）、（41.47±6.68）、（55.38±10.12）μmol/L,其中化合物1的抑制活性较强。结论 化合物1为新天然产物，化合物2～7为首次从灵芝属中分离得到。化合物1～2、4～7具有α-葡萄糖苷酶抑制活性。更多还原

【Abstract】 AIM To study the chemical constituents from Ganoderma angustisporum J.H. Xing, B.K. Cui & Y.C. Dai and their α-glucosidase inhibitory activities.METHODS The ethyl acetate extract from G. angustisporum was isolated and purified by silica gel, ODS, TLC and HPLC, then the structures of obtained compounds were identified by physicochemical properties and spectral data. pNPG method was used to evaluate their α-glucosidase inhibitory activities.RESULTS Seven compounds were isolated and identified as N-acetyl-L-phenylalanine ethyl ester（1）, N-acetyl-L-phenylalanine methyl ester（2）, 4-hydroxy-17R-methylincisterol（3）, 6,8-di-O-methylaverufin（4）, aversin（5）, methyl 2-（4-hydroxyphenyl） aceate（6）, 5-toluene-1,3-diol（7）. Compounds 1-2, 4-7 showed inhibitory activities of α-glucosidase with IC₅₀ values being（33.80±0.47）,（45.45±7.95）,（48.80±5.86）,（39.48±2.82）,（41.47±6.68）,（55.38±10.12） μmol/L, and compound 1 showed good inhibitory activity.CONCLUSION Compound 1 is a new natural product. Compounds 2-7 are isolated from genus Ganoderma for the first time. Compounds 1-2, 4-7 have α-glucosidase inhibitory activities.更多还原