【摘要】 2021—2022年，从中国福建省5个主要番茄种植区分离得到62株青枯病菌株（Ralstonia solanacearum）。通过演化型特异性多重聚合酶链式反应（phylotype-specificmultiplexpolymerasechain reaction,Pmx-PCR）、生理小种和生化型的鉴定，发现所有菌株均属于演化型Ⅰ和生理小种1，其中61株为生化型Ⅲ，1株为非典型生化型。基于egl基因部分序列的系统发育分析进一步将演化型Ⅰ细分为序列变种14、15、16、17和34，其中序列变种15是主要优势变种。对13个番茄砧木品种进行了青枯菌抗性评估，结果显示，不同砧木品种对不同序列变种的青枯菌表现出明显的抗性差异，‘桂砧1号’和‘威尔神根’对福建省的优势菌系表现出广谱抗性。更多还原

【Abstract】 In this study,62 strains of Ralstonia solanacearum were isolated in 2021 to 2022 from five tomato-growing areas in Fujian,China. Using phylotype-specific multiplex PCR(Pmx-PCR),the biochemical and race tests,all strains were identified as phylotype Ⅰ and race 1. Of these,61 strains were identified as belonging to biovar Ⅲ,while one strain was classified as atypical biovar. The strains were further divided five different sequevars(14,15,16,17,and 34)based on polymorphisms in the endoglucanase(egl)gene. Among the five sequevars,sequevar 15 was the predominant type in Fujian Province. In addition,the resistance of 13 tomato rootstock cultivars to R. solanacearum was evaluated,revealing significant differences in resistance among the cultivars to different sequence types of the pathogen. It is noteworthy that two rootstock cultivars,‘Guizhen 1’and‘Weier Shengen’,exhibited broad-spectrum resistance to the predominant strains in Fujian Province.更多还原