【摘要】 为了探索苹果轮纹病生物防治方法，分析了蜡样芽孢杆菌菌株G3-17挥发性有机化合物对苹果轮纹病菌的拮抗活性。平皿对扣试验显示，在前期研究基础上菌株G3-17不同浓度的挥发物均能显著抑制苹果轮纹病菌的生长。顶空固相微萃取—气相色谱—质谱联用分析显示，菌株G3-17主要分泌6种挥发性有机化合物，其中苯甲醛对苹果轮纹病菌的抑制效果最好，在浓度为125～625μL·L^-1范围内抑制率为57.24%～100.00%。苯甲醛处理还能破坏苹果轮纹病菌细胞膜的完整性，并能诱导苹果果实病程相关蛋白基因（Md PR1和Md PR5）的表达。防效试验结果显示，苯甲醛处理能有效防治苹果轮纹病，在浓度为125、250和500μL·L^-1时的防效分别为39.80%、84.77%和90.67%。研究表明，蜡样芽孢杆菌菌株G3-17挥发物及主要成分苯甲醛对苹果轮纹病具有较好的防治潜力。更多还原

【Abstract】 The purpose of this study is to analyze the antagonistic activity of volatile organic compounds（VOCs）of Bacillus cereus strain G3-17 against Botryosphaeria dothidea,and to explore the biological control methods of apple ring rot.The plate-to-plate experiment showed that the volatiles of Bacillus cereus strain G3-17 at different concentrations could significantly inhibit the growth of Botryosphaeria dothidea.Headspace solid-phase microextraction-gas chromatography-mass spectrometry analysis showed that the strain G3-17 mainly secreted six VOCs.Among them,benzaldehyde had the best inhibitory effect on Botryosphaeria dothidea,with an inhibition rate of 57.24%–100.00%in the concentration range of 125–625μL·L^-1.Further results also showed that benzaldehyde treatment also destroyed the integrity of the cell membrane of Botryosphaeria dothidea.In addition,benzaldehyde treatment could induce the expression of apple fruit pathogenesis-related protein genes（Md PR1 and Md PR5）.Benzaldehyde treatment also could effectively control apple ring rot caused by Botryosphaeria dothidea,and the control effects were 39.80%,84.77%and 90.67%at the concentrations of 125,250 and500μL·L^-1,respectively.In summary,the volatiles of Bacillus cereus strain G3-17 and its main VOCs have good control potential against apple ring rot.更多还原