【摘要】 目的 探讨固本安神方对围绝经期综合征（PMS）模型大鼠性激素水平及卵巢雌激素受体蛋白表达水平的影响。方法 选取动情周期规则的雌性SD大鼠60只，腹腔注射160 mg/kg去氧乙烯基环己烯（VCD），以复制PMS大鼠模型。造模成功后，将雌性SD大鼠随机分为模型组（B组，等体积生理盐水），中药组[C组，0.56 g/（kg·d）更年安片]，西药组[D组，0.1 mg/（kg·d）戊酸雌二醇片]，固本安神方高、中、低剂量组[E₁组、E₂组、E₃组，18.0,9.0,4.5 g/（kg·d）]，各10只。另取雌性SD大鼠10只为正常组（A组，等体积生理盐水）。各组大鼠分别灌胃给药4周后，摘取其子宫、双侧卵巢，称定湿重，计算子宫指数、卵巢指数；采用酶联免疫吸附试验（ELISA）法测定血清中卵泡刺激素（FSH）、促黄体生成素（LH）、雌二醇（E₂）的水平；采用免疫印记（Western blot）法测定卵巢组织中雌激素受体α（ERα）、雌激素受体β（ERβ）、G蛋白偶联雌激素受体1（GPER1）蛋白表达水平。结果 与B组比较，C组、D组、E₁组大鼠的子宫指数、卵巢指数均显著升高（P<0.01）;C组、D组、E₁组大鼠血清中FSH和LH水平均显著降低（P<0.01）,E₂水平均显著升高（P<0.01）;C组、D组、E₁组大鼠卵巢组织中ERα,ERβ,GPER1蛋白表达水平均显著升高（P<0.01）,E₂组、E₃组大鼠卵巢组织中ERα蛋白表达水平均显著升高（P<0.05）,E₂组大鼠卵巢组织中GPER1蛋白表达水平显著升高（P<0.05）。结论 固本安神方治疗PMS模型大鼠的作用机制可能与影响性激素分泌及提高卵巢雌激素受体的蛋白表达水平有关。更多还原

【Abstract】 Objective To investigate the effect of Guben Anshen Formula on sex hormone levels and ovarian estrogen receptor protein expression levels in perimenopausal syndrome （PMS） model rats.MethodsA total of 60 female SD rats with regular estrous cycles were selected and intraperitoneally injected with 160 mg/kg deoxyethylcyclohexene （VCD） to replicate the PMS model in rats.After successful modeling,60 female rats were randomly divided into the model group （group B,equal volume of normal saline）,traditional Chinese medicine group[group C,0.56 g/（kg·d） of Gengnian’an Tablets],Western medicine group[group D,0.1 mg/（kg·d） of Estradiol Valerate Tablets],and Guben Anshen Formula high-,medium-,and low-dose groups (groups E₁,E₂,E₃,18.0,9.0,4.5 g/（kg·d）],with 10 rats in each group.Another 10 female rats were selected as the normal group （group A,equal volume of normal saline）.After four weeks of gavage administration,the uterus and bilateral ovaries of rats in each group were removed and weighed with wet weight to calculated the uterine and ovarian indexes.Enzymelinked immunosorbent assay （ELISA） was used to determine the levels of serum follicle stimulating hormone （FSH）,luteinizing hormone （LH）,and estradiol （E₂）.Western blot was used to detect the expression levels of estrogen receptor α （ERα）,ERβand G-protein coupled estrogen receptor 1 （GPER1） proteins in ovarian tissues.ResultsCompared with those in group B,the uterine and ovarian indexes in groups C,D,and E₁were significantly higher （P<0.01）;the levels of serum FSH and LH were significantly lower （P<0.01）,while the level of E₂was significantly higher in groups C,D,and E₁（P<0.01）;the expression levels of ERα,ERβ,and GPER1 proteins in the ovarian tissues in groups C,D,and E₁were significantly higher （P<0.01）,the expression levels of ERα protein in the ovarian tissues in groups E₂and E₃were significantly higher （P<0.05）,and the expression levels of GPER1 protein in the ovarian tissues in group E₂were significantly higher （P<0.05）.ConclusionThe mechanism of Guben Anshen Formula in the treatment of PMS model rats may be related to its influence on sex hormone secretion and the increase of expression levels of ovarian estrogen receptor proteins.更多还原