【摘要】 目的 分析失笑散的化学成分。方法 采用超高效液相色谱串联三重四极杆飞行时间质谱（UPLC-Q-TOF-MS）法，色谱柱为Cortecs UPLC T3柱（100 mm×2.1 mm,1.6μm），流动相为0.1%甲酸水溶液-乙腈（梯度洗脱），流速为0.3 mL/min，柱温为30℃，进样量为2μL；电喷雾离子源（ESI），正负离子模式。结果 共解析出53个化学成分，其中组方药材蒲黄中23个，五灵脂中14个，其余16个为两者共有。蒲黄独有成分及其与五灵脂共有成分均以黄酮类成分为主，而五灵脂独有成分以萜类成分为主。结论 该方法操作简便，重复性好，可为进一步研究失笑散的效应-物质基础提供依据。更多还原

【Abstract】 Objective To analyze the chemical constituents of Shixiao Powder.MethodsUltra-high-performance liquidchromatography-triple quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was adopted. Thechromatographic column was Cortecs UPLC T3 column(100 mm × 2. 1 mm,1. 6 μm),the mobile phase was 0. 1% formic acid aqueous solution-acetonitrile(gradient elution),the flow rate was 0. 3 m L/min,the column temperature was 30 ℃,the injectionvolume was 2 μL,and the electro-spray ionization(ESI)was adopted with the positive and negative ion modes.ResultsA total of53 chemical constituents were obtained,including 23 in Typhae Pollen,14 in Faeces Trogopteror,and 16 were common in the twoChinese medicinal materials. The characteristic constituents of Typhae Pollen and the common constituents of the two Chinesemedicinal materials were mainly flavonoids,while the characteristic constituents of Faeces Trogopteror were mainly terpenoids.ConclusionThis method is easy and repeatable,which can provide a basis for the further research of the effect-material basisof Shixiao Powder.更多还原