【摘要】 目的 探讨miR-198对肝癌细胞株增殖和迁移的影响。方法 采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction, RT-qPCR)法检测miR-198在肝癌的不同细胞株中的表达量，然后运用细胞实验及动物实验进行miR-198对Huh7细胞株增殖、迁移及成瘤能力的研究。结果 RT-qPCR检测结果显示，与Chang liver和LO2正常肝细胞株比较，HepG2、Huh7、Hep3B和MHCC97H肝癌细胞株中的miR-198表达量降低，其中在Huh7细胞中尤为明显；流式细胞术检测结果显示，miR-198能够促进Huh7细胞凋亡；CCK-8及软琼脂克隆形成实验结果显示，与对照组比较，Huh7肝癌细胞株在转染miR-198模拟物后其增殖能力显著降低；划痕实验及Transwell实验检测结果显示，miR-198能够抑制Huh7肝癌细胞株的迁移能力；裸鼠成瘤实验结果显示，转染miR-198模拟物的Huh7肝癌细胞株的成瘤能力受到明显抑制。结论 miR-198在HepG2、Huh7、Hep3B和MHCC97H肝癌细胞株中的表达量明显降低，且在Huh7肝癌细胞株中最低；miR-198能够抑制Huh7肝癌细胞株增殖及迁移能力，降低其成瘤功能。更多还原

【Abstract】 Objective To investigate the effects of miR-198 on proliferation and migration of hepatocellular carcinoma cell lines. Methods RT-qPCR was used to detect the expression of miR-198 in different hepatocellular carcinoma cell lines, and the proliferation, migration and tumorigenic ability of miR-198 on Huh7 cell lines were identified by in cell experiments and in animal experiments. Results RT-qPCR test results showed that compared with Chang liver and LO2 normal liver cell lines, the expression levels of miR-198 in HepG2, Huh7, Hep3B and MHCC97H hepatocellular carcinoma cell lines were decreased, especially in Huh7 cells. Flow cytometry was used to confirm that miR-198 promoted the apoptosis of Huh7 cells. CCK-8 and soft agar clone formation experiment showed that compared with the control group, the proliferation of Huh7 hepatocellular carcinoma cell line after transfection with miR-198mimics was significantly reduced. The results of scratch test and Transwell test showed that miR-198 could inhibit the migration ability of Huh7 hepatocellular carcinoma cell line. The tumor formation experiment in nude mice proved that the tumor-forming ability of Huh7 hepatocellular carcinoma cell line transfected with miR-198 was obviously inhibited. Conclusion The expression level of miR-198 in HepG2, Huh7, Hep3B and MHCC97H hepatocellular carcinoma cell lines was significantly decreased, and the lowest expression was found in Huh7 hepatocellular carcinoma cell line. MiR-198 can inhibit the proliferation and migration ability of Huh7 hepatocellular carcinoma cell line, and reduce its tumor-forming ability.更多还原