【摘要】 目的：探讨程序性死亡受体1(PD-1)、程序性死亡配体1(PD-L1)、错配修复(MMR)蛋白(MLH1、PMS2、MSH2和MSH6)在子宫内膜癌中的表达及与患者临床病理特征的相关性。方法：选取我院2020年6月—2023年6月收治的80例子宫内膜癌患者作为研究对象，取患者的子宫内膜组织进行免疫组化检测，检测PD-1/PD-L1、MMR蛋白表达水平。并分析不同病理特征子宫内膜癌患者PD-1、PD-L1表达情况与MMR蛋白缺失情况，最后采用Spearman相关法分析PD-1、PD-L1、MMR蛋白与子宫内膜癌临床病理特征的相关性。结果：80例子宫内膜癌患者中，子宫内膜癌组织PD-1阳性表达率为63.75%,PD-L1阳性表达率为67.50%;MMR蛋白表达缺失率为70.00%;80例子宫内膜癌患者中，PD-1、PD-L1阳性与阴性患者的细胞分化程度、FIGO分期、子宫肌层浸润、淋巴结转移情况对比差异显著，此外PD-L1阳性与阴性病理类型对比差异显著(P<0.05),MMR蛋白基因缺失与基因完整患者的肿瘤部位、细胞分化程度、子宫肌层浸润、淋巴结转移情况对比差异显著(P<0.05);PD-1与子宫内膜癌细胞分化程度、FIGO分期、子宫肌层浸润、淋巴结转移呈正相关，PD-L1与病理类型、细胞分化程度、FIGO分期、子宫肌层浸润、淋巴结转移呈正相关，MMR蛋白与子宫内膜癌肿瘤部位、细胞分化程度、子宫肌层浸润、淋巴结转移呈正相关(P<0.05)。结论：PD-1、PD-L1、MMR蛋白表达与子宫内膜癌患者的临床病理特征具有密切关系，临床可考虑应用三者对子宫内膜癌的诊断与预后进行评价，为临床提供参考意义。更多还原

【Abstract】 Objective:Explore the expression of programmed death receptor 1(PD-1), programmed death ligand 1(PD-L1), mismatch repair(MMR) protein(MLH1,PMS2, MSH2 and MSH6) in endometrial cancer and its correlation with the clinicopathological characteristics of patients.Methods:80 cases of endometrial cancer patients admitted to our hospital from June 2020 to June 2023 were selected as research objects. Immunohistochemical detection was performed on endometrial tissues of patients to detect PD-1/PD-L1 and MMR protein expression levels. And analyze the expression of PD-1, PD-L1 and MMR protein deficiency in patients with endometrial cancer with different pathological characteristics. Spearman correlation method was used to analyze the correlation between PD-1, PD-L1, MMR protein and clinical pathological characteristics of endometrial cancer.Results:Among 80 patients with endometrial cancer, the positive expression rate of PD-1 and PD-L1 in endometrial cancer tissues was 63.75% and 67.50% respectively. The deletion rate of MMR protein expression was 70.00%. Among 80 patients with endometrial carcinoma, there were significant differences in the degree of cell differentiation, FIGO stage, myometrium infiltration and lymph node metastasis between PD-1 and PD-L1 positive and negative patients, and significant differences in the pathological types between PD-L1 positive and negative patients(P<0.05). There were significant differences in tumor site, cell differentiation, myometrial invasion and lymph node metastasis between patients with MMR protein gene deletion and patients with intact gene(P<0.05). PD-1 was positively correlated with the differentiation degree of endometrial cancer cells, FIGO stage, uterine infiltration, and lymph node metastasis; while PD-L1 was positively correlated with pathological type, cell differentiation degree, FIGO stage, uterine infiltration and lymph node metastasis; MMR protein was positively correlated with tumor site, cell differentiation, myometrium invasion and lymph node metastasis(P<0.05).Conclusion:The expression of PD-1, PD-L1 and MMR proteins is closely related to the clinicopathological features of patients with endometrial cancer. The three proteins can be considered for clinical evaluation of the diagnosis and prognosis of endometrial cancer to provide clinical reference significance.更多还原