【摘要】 目的 研究西双版纳西印度醋栗（Phyllanthus acidus）果实的化学成分及其抗乙肝病毒（HBV）活性和胰脂肪酶抑制活性。方法 综合运用大孔吸附树脂、Silica gel、Sephadex LH-20与半制备HPLC等多种色谱分离技术对西印度醋栗果实中的化学成分进行分离纯化，通过HRESIMS、IR、1D和2D NMR、ECD计算等现代波谱学方法进行结构鉴定。此外，分别使用HepG2.2.15细胞系与猪胰脂肪酶评估所得化合物的抗乙肝病毒（HBV）活性与脂肪酶抑制活性。结果 从西印度醋栗果实中分离得到13个化合物，包括1个新的没药烷型倍半萜（1）。其余化合物2-4为降没药烷型倍半萜，化合物5为杜松烷型倍半萜，化合物6-8为黄酮类化合物，化合物9为二萜，化合物10为生物碱，化合物11-13为木脂素衍生物。活性测试结果显示，上述化合物均未表现出明显地抗HBV活性（30μM）和胰脂肪酶抑制活性（200μM）。结论 本研究得到的化合物均首次从西印度醋栗果实中分离得到，并且化合物1为新的没药烷型倍半萜。并评估了所有化合物的抗HBV活性及胰脂肪酶抑制活性，为该植物资源进一步的开发与利用提供参考和科学依据。更多还原

【Abstract】 Objective To investigate the chemical constituents from the fruits of Phyllanthus acidus, and their antihepatitis B virus(HBV) activities and pancreatic lipase inhibitory activities. Methods The chemical constituents isolated from the fruits of Phyllanthus by chromatographic separation techniques, including macroporous adsorption resin, MCI, Silica gel, Sephadex LH-20, and semi-preparative HPLC. The structures of the compounds were identified by HRESIMS, IR, 1D and 2D NMR, and ECD calculations. Additionally, the anti-HBV activities and the pancreatic lipase inhibitory activities of the obtained compounds were tested using the HepG2.2.15 cells and porcine pancreatic lipase, respectively. Results Thirteen compounds were isolated from the fruits of Phyllanthus acidus, including one novel norbisabolane sesquiterpenoid(1). The remaining compounds were identified as norbisabolane sesquiterpenoids(2-4), a cadinane sesquiterpene(5),flavonoids(6-8), a diterpene(9), an alkaloid(10), and lignan derivatives(11-13). The results of activity test showed that no obvious anti-HBV activity(at 30 μM) and pancreatic lipase inhibition activity(at 200 μM). Conclusion All the compounds were obtained from the fruits of Phyllanthus acidus for the first time, and compound 1 was identified as a new norbisabolane sesquiterpenoid. The anti-HBV activities and pancreatic lipase inhibitory activities of all compounds were tested, which provided a reference and scientific basis for the further development and utilization of this plant resource.更多还原