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土壤熏蒸对三七连作土壤微生物群落的影响

Effects of Soil Fumigation on Microbial Community under Continuous Cropping of Panax notoginseng

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【作者】 王豪吉陈鹏崟王勇魏富刚杨绍周官会林徐武美

【Author】 WANG Haoji;CHEN Pengyin;WANG Yong;WEI Fugang;YANG Shaozhou;GUAN Huilin;XU Wumei;Yunnan Provincial Observation and Research Station of Soil Degradation and Restoration for Cultivating Plateau Traditional Chinese Medicinal Plants, Yunnan Normal University;Wenshan Sanqi Institute of Science and Technology, Wenshan University;Wenshan Miaoxiang Sanqi Technology Co.,Ltd.;

【通讯作者】 徐武美;

【机构】 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站文山学院文山三七研究院文山苗乡三七科技有限公司

【摘要】 用氯化苦熏蒸三七连作土壤,分析土壤微生物群落,并与林下健康三七种植土壤相比较,探究氯化苦熏蒸消减三七连作障碍的微生物学机理.结果表明:三七种植18个月后,试验组三七存活率为63.0%,而对照组三七存活率仅为2.0%.高通量测序与生信分析表明:与林下健康三七种植土壤相比,田间三七连作导致土壤微生物多样性降低(P<0.05),镰刀菌属(Fusarium)、癣囊腔菌属(Plectosphaerella)和链格孢属(Alternaria)等潜在病原菌群的相对丰度升高(P<0.05),伯克霍尔德菌属(Burkholderia)和慢生根瘤菌属(Bradyrhizobium)等潜在有益菌群的相对丰度降低(P<0.05).氯化苦熏蒸可有效降低三七连作土壤中病原菌群的相对丰度(P<0.05),调节微生物群落,显著提高连作三七存活率,是消减三七连作障碍,促进三七可持续栽培的有效措施.

【Abstract】 To investigate the microbiological mechanism of chloropicrin fumigation(CF) in reducing continuous cropping obstacles of Panax notoginseng,the soil microbial communities under CP were analyzed and compared with the healthy soil cultivating P. notoginseng under forests.The results showed that after 18 months of cultivation, the survival rate of P. notoginseng in the experimental group was 63.0%,while the survival rate of P. notoginseng in the control group was only 2.0%.High throughput sequencing and bioinformatic analyses showed that compared with the soil under forest, the continuous cropping of P. notoginseng led to the decrease of soil microbial diversity(P<0.05),moreover, the relative abundance of pathogenic microorganisms such as Fusarium,Plectosphaerella,and Alternaria was increased, and the beneficial Burkholderia and Bradyrhizobium was decreased(P<0.05).CF reduced the relative abundance of pathogens in soil(P<0.05),regulated microbial community, and improved the survival rate of P. notoginseng under continuous cropping, which is an effective measure to reduce continuous cropping obstacle and promote sustainable cultivation of P. notoginseng.

【基金】 国家自然科学基金资助项目(31901126);云南省“兴滇英才支持计划”资助项目(XDYC-QNRC-2022-0247);云南省院士专家工作站资助项目(202305AF150027,YSZJGZZ-2021062)
  • 【文献出处】 云南师范大学学报(自然科学版) ,Journal of Yunnan Normal University(Natural Sciences Edition) , 编辑部邮箱 ,2024年01期
  • 【分类号】S567.236;S154.3
  • 【下载频次】150
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