【摘要】 烟草根系分泌物中的酚酸类成分在土壤中过度积累是导致烟草连作障碍出现的重要原因.为减轻烟草连作障碍，以云南省昆明市连作烟田的土壤为材料，筛选出对烟草具有促生效果的酚酸降解菌N8.通过形态学和分子生物学方法确定该菌为短波单胞菌（Brevundimonas naejangsanensis）.该菌株可降解烟草连作土壤中73.4%的阿魏酸和83%的苯甲酸.连作土盆栽烟草经该菌株处理后，其株高、茎围、叶长指标相比于对照组，分别增长了17.22%、16.86%、5.05%.在田间小区试验中，N8菌株处理组烟草的株高、干重指标均高于对照组，分别提高2.13%与23.33%.研究筛选出的菌株N8为消除烟草连作障碍提供了有益的菌株储备.更多还原

【Abstract】 The excessive accumulation of phenolic acids in tobacco root is an important reason for the occurrence of tobacco continuous cropping disorder. To alleviate the barriers to tobacco continuous cropping, a phenol acid-degrading strain N8 was isolated from the soil of continuous cropping tobacco field in Kunming,Yunnan Province. The strain was identified as Brevundimonas naejangsanensis by morphological and molecular biological methods. This strain could degrade 73.4% of ferulic acid and 83% of benzoic acid in tobacco continuous cropping soil. Compared with control group cultiveated in continuous croping soil in pot, plant height, stem circumference, and leaf length of tobacco treated by N8 strain increased by 17.22%, 16.86%, and 5.05%. In the field plot experiment, plant height and dry weight of tobacco in the N8 treatment group were higher than those in the CK control group, increased by 2.13% and 23.33%, respectively. Strain N8 screened in this study provided a beneficial strain reserve for eliminating tobacco continuous cropping barriers.更多还原