【摘要】 采用超声波辅助普通法和超声波辅助酶法对库页红景天进行提取，并对比分析了提取液中Mn、Fe、Cu、Zn4种微量元素的形态.通过D101大孔树脂分离，将库页红景天提取液中的Mn、Fe、Cu、Zn分为无机态、有机态、黄酮类结合态、丙酮分离蛋白态和乙醇分离多糖态.利用电感耦合等离子质谱法（ICP-MS）测定了各形态中4种微量元素的含量.测量结果表明，在加入纤维素酶和复合酶进行提取时，无机态和有机态中的4种微量元素含量显著提高，且黄酮类结合态含量也有所增加.而单独加入纤维素酶提取时，多糖态中的Cu和Zn含量有所降低.此外，4种微量元素的形态分布在加入纤维素酶和复合酶后也发生了变化.这些研究结果为提高库页红景天中微量元素的生物活性和利用度提供了重要的参考依据.更多还原

【Abstract】 Ultrasound-assisted conventional extraction and ultrasound-assisted enzymatic extraction methods were employed to extract Rhodiola saccharinensis,and the speciation of Mn,Fe,Cu,and Zn in the extracts was comparatively analyzed.Through separation using D101 macroporous resin,Mn,Fe,Cu,and Zn in the Rhodiola saccharinensis extracts were categorized into inorganic,organic,flavonoid-bound,acetone-separated protein-bound,and ethanol-separated polysaccharide-bound forms. Inductively coupled plasma mass spectrometry(ICP-MS) was utilized to determine the content of the four trace elements in each form. The measurement results indicated that the content of the four trace elements in the inorganic and organic forms significantly increased when cellulase and a composite enzyme were added for extraction,and the content of flavonoid-bound forms also increased. However,when cellulase was added alone for extraction,the content of Cu and Zn in the polysaccharide-bound forms decreased. Moreover,the speciation distribution of the four trace elements also changed. These findings provide important reference for enhancing the bioactivity and utilization of trace elements in Rhodiola saccharinensis.更多还原