【摘要】 目的：探讨白藜芦醇对晚期糖基化终产物（AGEs）诱导H9C2心肌细胞凋亡的保护作用。方法：体外培养H9C2细胞，实验分为4组：正常组、AGEs组、AGEs+白藜芦醇（RESV）组、AGEs+NAC组。通过CCK-8比色法检测细胞存活率，相差显微镜观察细胞形态，双氯荧光素（DCFH-DA）染色荧光显微镜摄片观察细胞内活性氧（ROS）水平；运用Hoechst33258检测试剂盒及流式细胞术检测心肌细胞的凋亡率，Western Blot检测相关促凋亡蛋白Bax、抑制凋亡蛋白Bcl-2表达量。结果：与正常组比较，AGEs组H9C2细胞生存率降低，ROS生成量增加（P<0.01）；与AGEs组比较，AGEs+RESV组细胞生成率提高，ROS生成量减少（P<0.01）。与正常组比较，AGEs组促凋亡蛋白Bax表达增加，抑制凋亡蛋白Bcl-2表达减少，细胞凋亡率升高（P<0.05）；与AGEs组比较，AGEs+RESV组可下调促凋亡蛋白Bax表达，上调凋亡抑制蛋白Bcl-2表达，细胞凋亡率降低（P<0.05）。结论：白藜芦醇对AGEs诱导的H9C2心肌细胞凋亡具有保护作用，其保护机制与减少ROS有关。更多还原

【Abstract】 Objective: To investigate the effect of trans-3,4,5-trihydroxystilbene on H9C2 cardiomyocytes against AGEs-induced apoptosis and potential molecular mechanisms. Methods: H9C2 cardiomyocytes cells cultured in vitro were divided into four groups:normal contol group, AGEs group, AGEs and trans-3,4,5-trihydroxystilbene(RESV) group, AGEs and NAC group. Cell viabillity rate was measured by CCK-8 assay,ROS production was measured by DCFH-DA fluorescent probe. Nucleus morphology was observed under fluorescence microscope after being incubatedwith Honchest 33258; western blotting was applied to assess the apoptotic components expression including Bax and Bcl-2. Results: Compared with control group, cell viability rate in AGEs group were decreased, repectively, in a dose-dependent manner; cell apoptosis and ROS production in H9C2 cells were remarkably increased in AGEs group(P<0.05). However, compared with AGEs group, trans-3,4,5-trihydroxystilbene group reduced ROS productionand ameliorated cell apoptosiscaused by AGEs(P<0.05);the expression of proapotosis proteins Bax was decreased, the expression of anti-apoptosis proteins like Bcl-2 was increased(P<0.05).Conclusion: Trans-3,4,5-trihydroxys tilbenne protect H9C2 cardiomyocytes against AGEs-induced apoptosis,which may be related to the reduction of ROS.更多还原