【摘要】 目的 分析胃癌组织中白细胞介素-32(Interleukin-32, IL-32)与凋亡蛋白酶激活因子-1(Apoptotic Protease Activating Factor-1, Apaf-1)的表达及意义。方法 回顾性选取2019年1月—2022年12月南通市肿瘤医院收治的100例胃癌患者的临床资料，分别取其胃癌组织标本（胃癌组）和正常胃黏膜标本（癌旁组），通过免疫组化法（Streptavidin-perosidase, SP）检测胃癌标本与正常标本中IL-32、Apaf-1蛋白的阳性表达情况。结果胃癌组IL-32阳性表达率高于癌旁组，Apaf-1蛋白阳性表达率低于癌旁组，差异有统计学意义（P均<0.05）；胃癌患者高中分化程度与低分化程度之间，浸润深度<肌层与≥肌层之间，TNM分期为Ⅰ、Ⅱ期与分期为Ⅲ、Ⅳ期之间以及有无淋巴结转移之间的IL-32、Apaf-1蛋白阳性表达率对比，差异有统计学意义（P均<0.05）。结论 IL-32与Apaf-1蛋白的阳性表达可为胃癌患者的临床诊断和治疗提供重要的参考价值。更多还原

【Abstract】 Objective To analyze the expression and significance of interleukin-32(IL-32) and apoptotic protease activating factor-1(Apaf-1) in gastric cancer tissues. Methods The clinical data of 100 gastric cancer patients admitted to Nantong Cancer Hospital was retrospectively selected, and their gastric cancer tissue specimens(gastric cancer group) and normal gastric mucosa specimens(paracarcinoma group) were taken respectively. The positive expression of IL-32 and Apaf-1 proteins in gastric cancer specimens and normal specimens were detected by immunohistochemistry streptavidin-perosidase(SP). Results The positive expression rate of IL-32 in the gastric cancer group was higher than that of paracarcinoma group, and the positive expression rate of Apaf-1 protein was lower compared with that of the paraneoplastic group, the differences were statistically significant(both P<0.05). There were statistically significant differences in IL-32 and Apaf-1 protein-positive expression rates between high and medium differentiation degree and low differentiation degree, between infiltration depth <muscular layer and ≥muscular layer, between TNM stage I and Ⅱ and stage Ⅲ and Ⅳ, and between with and without lymph node metastasis in gastric cancer patients(all P<0.05). Conclusion The positive expression of IL-32 and Apaf-1 protein can provide important reference value for clinical diagnosis and treatment of gastric cancer patients.更多还原