【摘要】 天然水凝胶支架由亲水性聚合物链交联形成，有相互连接的多孔结构、良好的生物相容性，通过模拟细胞外基质为细胞的增殖、分化、迁移和相互作用提供了空间微环境，是一种很有前景的3D细胞培养支架材料。本文从制备方法、交联方式、支架材料对天然水凝胶支架进行分类，详细介绍了近年来基于天然水凝胶支架的3D细胞培养在细胞工程、组织工程、病毒培养等生物医学领域的应用。随着生物医学不断发展，需深入研究3D培养细胞与天然水凝胶支架微环境之间相互作用，不断探索新的天然聚合物材料与凝胶制备方式，以便开发高性能、可调控、适用性强的多功能新型天然水凝胶。更多还原

【Abstract】 Natural hydrogel scaffolds are formed by cross-linking hydrophilic polymer chains with interconnected porous structure, good biocompatibility, and provide a spatial microenvironment for cell proliferation, differentiation, migration, and interactions by mimicking the extracellular matrix, which is a promising scaffolding material for 3D cell culture. This paper classifies natural hydrogel scaffolds in terms of preparation method, cross-linking method, and scaffold material, and describes in detail the application of 3D cell culture based on natural hydrogel scaffolds in recent years in biomedical fields such as cell engineering, tissue engineering, and viral culture. With the development of biomedical disciplines, the interaction between cells and microenvironment in 3D cell culture should be studied, and new natural polymer materials and preparation methods should be further explored in order to design multifunctional natural hydrogels with high performance, regulation and strong applicability.更多还原