【摘要】 甘蓝型油菜黄籽性状遗传来源较为复杂且易受环境条件影响，基因定位和分子标记开发难度大.相比于传统QTL定位，结合二代深度测序等生信方法直接对甘蓝型油菜黄籽基因进行染色体定位、基因挖掘和标记开发，将有助于黄籽遗传机理的研究.利用甘蓝型油菜黄籽临保系63-2和黑籽67-1配制杂交组合，构建F₂粒色分离群体.遗传分析表明：该群体中黄籽性状受1对隐性主效基因控制；以法国甘蓝型油菜Darmor-bzh基因组为参考，利用集团分离分析法和全基因组重测序将黄籽性状基因定位于C03染色体6.1～9.0 Mb置信区间内，同时基于基因组变异和聚丙烯酰胺凝胶电泳验证获得与粒色基因紧密连锁的SSR标记4个和InDel标记5个.更多还原

【Abstract】 The genetic inheritance of yellow-seed trait is complex and easily affected by environmental factors in Brassica napus. The gene mapping and linked markers developing are very difficult for yellow-seeded rapeseed. Compared with traditional QTL mapping, we carried out the next generation deep sequencing to perform the gene mapping and identification of chromosome, and marker developing for yellow-seed gene, which will be helpful to reveal the genetic mechanism of yellow-seed trait in rapeseed. In this study, we constructed a F₂ mapping population from the cross between yellow-seeded line 63-2 and black-seeded line 67-1. Genetic analysis showed that yellow-seed trait was controlled by a pair of recessive major genes. The bulked segregant analysis（BSA） combining with whole-genome resequencing was employed to mine the candidate region of the chromosome for controlling the yellow-seed trait, which was anchored on chromosome C03（6.1～9.0 Mb） based on the Darmor-bzh reference genome. Eventually, four SSR markers and five InDel markers closely linked with the yellow-seed trait were screened and further confirmed by polyacrylamide gel electrophoresis（PAGE） analysis.更多还原