【摘要】 【目的】研究4-香豆酸辅酶A连接酶(4CL)家族基因Gh4CL30的生物学功能，为棉花株型育种提供理论依据和基因种质资源。【方法】利用病毒诱导的基因沉默技术和基因编辑技术获得Gh4CL30沉默和编辑植株，测定该基因抑制及敲除植株的黄酮和木质素含量，调查棉花田间表型性状、种子大小和纤维品质。【结果】Gh4CL30基因沉默和敲除植株中木质素合成相关基因表达量显著下降，4-香豆酸辅酶A连接酶含量显著减少，茎秆中木质素含量显著降低，其株高、种子大小和纤维长度显著降低。【结论】Gh4CL30通过调控棉花木质素生物合成功能影响其生长发育。更多还原

【Abstract】 【Objective】 To preliminarily study the biological function of 4-coumarate-CoA ligase(4CL) family gene gh4cl30, which might provide theoretical basis and genetic resources for cotton plant breeding.【Methods】 Virus-induced gene silencing technology and gene editing technology was used to obtain gh4cl30-silenced and edited plants and determine the flavone and lignin contents of the gene suppressed and knockout plants to investigate field phenotype, seed size and fiber quality.【Results】 Both gene silencing and gene editing of the gene resulted in dwarfing of the plants and a significant reduction of lignin content in the plant stalks.Field phenotypic measurements showed that seed size and fiber length were significantly reduced in the gene edited plants.【Conclusion】 Gh4CL30 affects cotton growth and development mainly through regulating lignin biosynthesis.更多还原