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基于KASP技术鉴定新疆小麦抗病性相关基因

Identification of disease resistance of Xinjiang wheat based on KASP technology

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【作者】 高新时佳王春生王重李剑峰李艾玲樊哲儒张跃强

【Author】 GAO Xin;SHI Jia;WANG Chunsheng;WANG Zhong;LI Jianfeng;LI Ailing;FAN Zheru;ZHANG Yueqiang;Key Laboratory of Desert-Oasis Crop Physiology,Ecology and Tillage,MOAR/Research Institute of Nuclear and Biological Technology,Xinjiang Academy of Agricultural Sciences/Xinjiang Key Laboratory of Crop Biotechnology/Xinjiang Crop Chemical Regulation Engineering Technology Researching Center;School of Science and Technology,Xinjiang Agricultural University;

【通讯作者】 樊哲儒;张跃强;

【机构】 新疆农业科学院核技术生物技术研究所/农业部荒漠绿洲作物生理生态与耕作重点实验室/新疆农作物生物技术重点实验室/新疆作物化学调控工程技术研究中心新疆农业大学科学技术学院

【摘要】 【目的】鉴定新疆小麦审定品种(系)抗病性相关基因,为小麦育种遗传改良提供材料。【方法】利用KASP技术对183份新疆小麦品种(系)的抗病性相关基因进行检测。【结果】99份材料携带抗叶锈病基因Lr46,频率为54.10%,64份材料携带抗叶锈病基因Lr14a,频率为34.97%,3份材料携带抗叶锈病基因Lr68,0份材料携带抗白粉病基因Pm21。新疆小麦品种(系)中抗病基因的分布频率较低,约为26.8%。【结论】筛选出的146份材料可以作为新疆小麦持久抗锈病育种的抗源材料。

【Abstract】 【Objective】 This study aims to identify the genes related to disease resistance of Xinjiang wheat varieties(lines) and provide materials for genetic improvement of wheat breeding.【Methods】 In the present study, KASP technology was used to detect the genes related to disease resistance in 183 Xinjiang wheat varieties(lines).【Results】 The results showed that 99 varieties had the leaf rust resistance gene Lr46 with a frequency of 54.10%,64 varieties had the leaf rust resistance gene Lr14a with a frequency of 34.97%,3 varieties had the leaf rust resistance gene Lr68,no variety had the powdery mildew resistance gene Pm21 and yellow rust resistance gene Yr15.The distribution frequency of resistance genes in Xinjiang wheat varieties(lines) was low with a frequency of about 26.8%.【Conclusion】 The 146 selected materials could be used as resistance materials for wheat breeding of long term rust resistance in Xinjiang.

【关键词】 小麦抗病性KASP标记检测
【Key words】 wheatdisease resistanceKASPmark detection
【基金】 新疆生产建设兵团农业核心攻关项目(NYHXG-2023AA201);新疆农作物生物技术重点实验室开放课题(XNYPT-2021-004);新疆现代农业小麦产业技术体系(XJARS-01)~~
  • 【文献出处】 新疆农业科学 ,Xinjiang Agricultural Sciences , 编辑部邮箱 ,2024年03期
  • 【分类号】S512.1
  • 【下载频次】72
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