【摘要】 目的：探究枸杞多糖（LBP）和亚硒酸钠（Na₂SeO₃）对镉（Cd）致大鼠肝毒性的拮抗作用。方法：40只SD大鼠随机分为对照组、CdCl₂组、LBP组、Na₂SeO₃组、LBP联合Na₂SeO₃组，每组8只。对照组不造模，其余四组腹腔注射CdCl₂染毒。染毒1 h后，对照组和CdCl₂组给予生理盐水灌胃，LBP组、Na₂SeO₃组分别给予LBP、Na₂SeO₃灌胃，LBP联合Na₂SeO₃组给予LBP和Na₂SeO₃灌胃，每日染毒和灌胃各进行1次，共干预35 d。观察各组大鼠肝脏病理改变，测定大鼠肝组织Cd含量；检测血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、直接胆红素（DB）及总胆红素（TB）的含量以及肝组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）含量；Western blot检测肝组织中苹果酸脱氢酶（MDH）、琥珀酸脱氢酶（SDH）、铁调节蛋白（IRP-1）和谷胱甘肽过氧化物酶4（GPX4）的蛋白表达水平。结果：与CdCl₂组、LBP组和Na₂SeO₃组相比，LBP联合Na₂SeO₃组肝细胞排列更紧密，细胞核更清晰可见，肝小叶中央静脉无淤血。相比CdCl₂组，LBP联合Na₂SeO₃组能降低Cd在大鼠肝脏的蓄积（P<0.05）。LBP联合Na₂SeO₃组与CdCl₂组相比，血清ALT、AST水平降低（P<0.05）;LBP联合Na₂SeO₃组与CdCl₂组相比，大鼠肝脏组织中SOD、GSH-Px水平显著升高，MDA降低（P<0.05）;LBP联合Na₂SeO₃组大鼠肝脏组织中MDH、SDH、IRP-1、GPX4的蛋白表达水平较CdCl₂组、LBP组和Na₂SeO₃组升高（P<0.05）。结论：LBP和Na₂SeO₃联合干预可能通过增强大鼠肝脏的抗氧化能力拮抗Cd诱导的肝损伤。更多还原

【Abstract】 Objective:To explore the antagonistic effect of Lycium barbarum polysaccharide （LBP） and sodium selenite （Na₂SeO₃）on hepatotoxicity induced by cadmium （Cd） in rats.Methods:Forty SD rats were randomly divided into control group,CdCl₂group,LBP group,Na₂SeO₃ group and LBP combined Na₂SeO₃ group,with eight rats in each group.The control group was not molded,and the other four groups were infected by intraperitoneal injection of CdCl₂.1 h after exposure,the control group and CdCl₂ groupwere given normal saline intragastric administration.LBP group and Na₂SeO₃ group were given LBP and Na₂SeO₃ by gavage.LBPcombined with Na₂SeO₃ group was given LBP and Na₂SeO₃ by gavage.The patients were treated once a day with poison and oncea day with intragastric administration for 35 days.The liver pathological changes of each group were observed and the content ofCd in liver tissue was determined.The contents of alanine aminotransferase （ALT）,aspartate aminotransferase （AST）,directbilirubin （DB） and total bilirubin （TB） in serum and the contents of superoxide dismutase （SOD）,glutathione peroxidase （GSH-Px）and malondialdehyde （MDA） in liver tissue were detected.Western blot was used to detect the protein expression levels of malatedehydrogenase （MDH）,succinate dehydrogenase （SDH）,iron regulatory protein （IRP-1）,and glutathione peroxidase 4 （GPX4） inliver tissue.Results:Compared with CdCl₂ group,LBP group and Na₂SeO₃ group,the liver cells in LBP combined with Na₂SeO₃ group were more closely arranged,the nucleus was more clearly visible,and the central vein of the hepatic lobule was not stasis;compared with CdCl₂ group,LBP combined with Na₂SeO₃ group could reduce the accumulation of Cd in the liver of rats （P<0.05）;serum ALT and AST levels in LBP combined with Na₂SeO₃ group were lower than those in Cd Cl₂group （P<0.05）;compared with CdCl₂ group,the levels of SOD and GSH-Px in liver tissues of LBP combined with Na₂SeO₃ group were significantly increased,and MDA was decreased （P<0.05）;the protein expression levels of MDH,SDH,IRP-1 and GPX4 in liver tissues of rats in LBPcombined with Na₂Se O₃ group were higher than those in CdCl₂,LBP and Na₂SeO₃ groups （P<0.05）.Conclusion:Combinedintervention of LBP and Na₂SeO₃ may antagonize CD-induced liver injury by enhancing the antioxidant capacity of rat liver.更多还原