【摘要】 目的 研究蛇含委陵菜的抗炎活性部位及化学成分。方法 用二甲苯致小鼠耳肿胀急性炎症模型筛选蛇含委陵菜体积分数为60%的乙醇提取物的水部位（FPA）、体积分数为50%的乙醇部位（FPB）、体积分数为95%的乙醇部位（FPC）的抗炎活性。进一步用MCI、Sephadex LH-20、正相和反相硅胶等柱层析方法对活性部位的化学成分进行分离纯化，通过理化性质和核磁共振波谱对分离得到的化合物进行结构鉴定。结果 蛇含委陵菜FPB具有抗炎活性，并从该部位中分离得到10个化合物，分别鉴定为：补骨脂素（1）、邻苯二甲酸-双（2’-乙基庚基）酯（2）、毛两面针素（3）、附子亭碱（4）、1,2-二羟基蒽醌（5）、熊果苷（6）、山柰酚（7）、桔皮素（8）、异鼠李素（9）、金合欢素-7-O-α-L-吡喃鼠李糖-（1→6）-β-D-吡喃葡萄糖（10）。结论 蛇含委陵菜FPB是其抗炎活性部位;从该部位中分离鉴定了10个化合物，其中化合物2～4、7～10为首次从委陵菜属植物中分离得到，化合物1、6为首次从该植物中分离得到。更多还原

【Abstract】 Objective To screen the anti-inflammatory fractions of Potentilla kleiniana Wight. et Arn. and to study the chemical constituents from the active fractions. Methods The anti-inflammatory activity of water fraction(FPA), 50% ethanol fraction(FPB) and 95% ethanol fraction(FPC) of 60% ethanol extract Potentilla kleiniana Wight. et Arn. were evaluated by mice ear swelling acute inflammation model induced by xylene. The constituents from active fraction were further isolated by MCI, Sephadex LH-20, silica gel, octadecyl-silylated silica gel column chromatography and other methods, and their chemical structures were elucidated on the basis of physicochemical properties and spectral analysis. Results The 50% ethanol fraction exhibited anti-inflammatory activity, and 10 compounds were isolated and their structures were identified as: psoralen(1), bis(2’-ethylheptyl) phythalate(2), toddalolactone(3), fuzitine(4), 1,2-oxyanthraquinone(5), arbutin(6), kaemperol(7), tangeretin(8), isorhamnetin(9), and acacetin-7-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(10). Conclusion The 50% ethanol fraction is the antiinflammatory fraction, from which 10 compounds were separated and elucidated. Compouds 2—4, 7—10 were isolated from Potentilla genus for the first time, and compounds 1 and 6 were isolated from Potentilla kleiniana Wight. et Arn. for the first time.更多还原