【摘要】 为了研究带内生真菌(E+)和不带内生真菌(E-)布顿大麦(Hordeum bogdanii)的耐碱性，选择来源于新疆阿克苏温宿县的带内生真菌(E+)和不带内生真菌(E-)布顿大麦种子，先在培养皿中进行发芽，然后进行室内蛭石无土栽培，对其进行21 d的不同浓度混合碱胁迫处理，测定其渗透调节和膜系统变化。结果表明，在相同混合碱处理下，E-植株的相对电导率均显著高于E+植株(P<0.05),E+植株的细胞通透性弱于E-植株。在碱胁迫下E+植株的脯氨酸、可溶性糖和可溶性蛋白含量均显著高于E-植株(P<0.05)。内生真菌具有提高宿主渗透调节物质和降低膜损伤来提高布顿大麦抗碱的能力。更多还原

【Abstract】 This study aimed to investigate alkaline tolerance of Hordeum bogdanii barley, comparing plants with endophytic fungi(E+) and those without endophytic fungi(E-). Seeds of alkaline-tolerant barley from Aksu Wensu County in Xinjiang were used as experimental materials. Initially, the seeds were germinated in petri dishes and then cultivated in vermiculite soilless conditions in a laboratory setting. Over a 21-day period, the changes in osmotic regulation and membrane system under treatments of different mixed alkali concentration were observed. The results showed that E-plants exhibited significantly higher relative electrical conductivity compared to E+ plants when subjected to the same mixed alkali treatment(P<0.05), indicating greater cell membrane damage in E-plants.Conversely, the E+ plants exhibited stronger cell permeability and higher levels of proline, soluble sugar, and soluble protein under alkali stress compared to E-plants(P<0.05).In conclusion, Endophytic fungi play a role in enhancing the host’s osmotic regulation substances and reducing membrane damage, thereby improving alkali resistance of Hordeum bogdanii barley.更多还原