【摘要】 叶绿素荧光是反映植物光合效率的重要参数。叶绿素荧光成像技术能对植株光合作用二维空间异质性进行分析，然而现有的叶绿素荧光成像系统需要昂贵的相机和复杂的控制系统，为此提出一种基于低成本CMOS相机的叶绿素荧光成像方法。提出一种可变积分时间的叶绿素荧光图像接收方法，通过改变不同强度激发光下CMOS相机的积分时间，解决了低成本CMOS相机在荧光接收中的控制延迟问题；设计一种简易的叶绿素荧光成像系统，实现800万像素、成像面积为60mm×80mm的荧光图像采集。试验结果显示，该系统能够测量得到清晰的荧光图像，可以在1mm~2的区域检测到植物的光合异质性，将测量结果与商业荧光仪测得的结果相比，决定系数达到0.94。本研究提出的荧光成像方法在可行性和准确性方面取得较好的效果，成像系统测量结果稳定可靠、成本低廉，为叶绿素荧光成像技术提供了更为经济高效的解决方案。更多还原

【Abstract】 Chlorophyll fluorescence is an important parameter reflecting the photosynthetic efficiency of plants. Chlorophyll fluorescence imaging technology can analyze the three-dimensional spatial heterogeneity of plant photosynthesis. However, existing chlorophyll fluorescence imaging systems require expensive cameras and complex control systems. Therefore, this paper proposes a chlorophyll fluorescence imaging method based on low-cost CMOS cameras. A chlorophyll fluorescence image reception method with variable integration time is proposed. By changing the integration time of the CMOS camera under different intensities of excitation light, the control delay problem in fluorescence reception of low-cost CMOS cameras is addressed. A simple chlorophyll fluorescence imaging system is designed to achieve fluorescence image acquisition with 8 million pixels and an imaging area of 60mm×80mm.Experimental results show that the system can obtain clear fluorescence images and detect plant photosynthesis heterogeneity in a 1mm~2 area. Compared with the results measured by the PSI MP100 fluorometer, the determination coefficient reaches 0.94.The proposed fluorescence imaging method demonstrates good feasibility and accuracy. The imaging system provides stable and reliable measurement results at a low cost, offering a more economical and efficient solution to chlorophyll fluorescence imaging technology.更多还原