【摘要】 【目的】建立肉制品中牛、鸭源性成分的现场快速定量检测方法。【方法】将DNA免提取法与荧光定量PCR(qPCR)冻干试剂法相结合，采用DNA免提取法制备qPCR反应模板。通过冻干工艺制备出的预混qPCR试剂，利用便携式qPCR系统对冻干试剂进行扩增。【结果】DNA免提取法简化了样品的提取、纯化步骤，该过程可在10 min内完成。DNA免提取法结合qPCR冻干试剂法的检测限可达1 pg/μL，从采样到得出结果只需45 min。预混qPCR冻干试剂可在4℃下保存6个月、室温下保存4个月、37℃下保存14 d。【结论】利用便携式qPCR系统即可对qPCR冻干检测试剂进行扩增，从而实现肉制品掺假的快速鉴别。更多还原

【Abstract】 [Objective] This study aims to achieve rapid quantitative detection of beef-and duck-derived ingredients in meat products. [Method] The DNA extraction-free method was combined with fluorescent quantitative PCR(qPCR)lyophilized reagents was developed. The DNA extraction-free method was employed to prepare the template for qPCR.The premixed qPCR reagents were prepared by lyophilization. A portable qPCR system was used for amplification with the lyophilized reagents. [Result] The DNA extraction-free method with simplified sample extraction and purification steps achieved the detection within 10 min. The method combining the DNA extraction-free DNA method and qPCR lyophilized reagents can produce results within 45 min, with the limit of detection being 1 pg/μL. The premixed qPCR lyophilized reagents can be stored at 4 ℃ for 6 months, at room temperature for 4 months, and at 37 ℃ for 14 d.[Conclusion] The qPCR lyophilized reagents are appropriate for a portable qPCR system and can thus achieve rapid identification of adulteration in meat products.更多还原