【摘要】 为探究红糖的抗氧化活性及其成分，作者首先采用叔丁基过氧化氢（tert-butyl hydroperoxide,t-BHP）诱导的Caco-2细胞作为氧化损伤模型，以评估红糖提取物对细胞存活率、细胞内活性氧（reactive oxygen species,ROS）水平和细胞抗氧化能力（cellular antioxidant activity,CAA）的影响，并采用高效液相色谱串联质谱（HPLC-MS/MS）技术对红糖中的主要活性成分——酚类化合物进行分析。结果表明，红糖提取物能够改善由t-BHP造成的细胞凋亡，可显著降低细胞内ROS水平，具有一定的抗氧化活性，每克红糖提取物的CAA值相当于12.27μmol槲皮素。另外，红糖提取物中含有原儿茶酸、香草酸、绿原酸、氯化矢车菊素、香豆素和芹菜素等酚类化合物。该研究为红糖及红糖提取物的活性研究和相关功能性产品的开发提供了理论依据。更多还原

【Abstract】 To investigate the antioxidant activity of non-centrifugal sugar, tert-butyl hydroperoxide(t-BHP)-induced Caco-2 cells were used as the model of oxidative damage to assess the effects of non-centrifugal sugar crude extracts on cell viability, intracellular reactive oxygen species(ROS) levels, and cellular antioxidant activity(CAA). Additionally, high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) was employed to analyze the main active phenolic compounds in non-centrifugal sugar. The results demonstrated that the non-centrifugal sugar extract could alleviate apoptosis induced by t-BHP,significantly reduce intracellular ROS levels, and exhibit notable antioxidant activity. The CAA value of the non-centrifugal sugar extract was equivalent to 12.27 μ mol quercetin per gram of extract. Moreover, the noncentrifugal sugar extract contained phenols substances such as protocatechuic acid, vanillic acid, chlorogenic acid, cyanidin chloride, coumarin, and apigenin. This study provides a theoretical foundation for exploring the bioactivity of non-centrifugal sugar and its extracts, as well as the development of related functional products.更多还原