【摘要】 背景：结直肠癌的发病率和死亡率在全球均居前列。整合素β样蛋白1(ITGBL1)可发挥整合素样作用，在恶性肿瘤的发生、发展中具有重要意义。目的：探讨ITGBL1在结直肠癌组织和细胞中的表达和临床意义及其对结直肠癌细胞生物学功能的影响。方法：收集苏州大学附属第一医院2023年9月—2024年5月55例结直肠癌手术患者的癌组织和配对癌旁组织标本，以免疫组化染色检测ITGBL1蛋白表达，并分析其表达与患者临床病理特征的关系。利用sh-ITGBL1慢病毒系统敲减结直肠癌细胞的ITGBL1表达，以蛋白质印迹法检测ITGBL1蛋白表达，以CCK-8实验和克隆形成实验分析ITGBL1敲减对结直肠癌细胞增殖能力的影响。结果：ITGBL1在结直肠癌组织和细胞中均呈高表达；其表达与肿瘤浸润深度、淋巴结转移和临床分期呈显著正相关（P均<0.05）。与对照组相比，ITGBL1敲减结直肠癌细胞株HCT116、RKO中的ITGBL1表达降低，细胞增殖明显受抑（P均<0.05）。结论：ITGBL1高表达可能与结直肠癌的发生、发展有关。ITGBL1敲减对结直肠癌细胞增殖有明显抑制作用。ITGBL1有望成为结直肠癌诊断的潜在生物学标志物和新的治疗靶点。更多还原

【Abstract】 Background: Colorectal cancer(CRC) remains one of the leading causes of cancer incidence and mortality worldwide. As an integrin-like molecule, ITGBL1(integrin, beta-like 1) may play a crucial role in the tumorigenesis and progression of malignancies. Aims: To explore the expression of ITGBL1 in CRC tissues and cells, and its clinical significance and effect on the biological function of CRC cells. Methods: Fifty-five pairs of paraffin-embedded cancerous and paracancerous tissues from 55 CRC patients undergoing surgical treatment from September 2023 to May 2024 at the First Affiliated Hospital of Soochow University were collected. Protein expression of ITGBL1 was determined by immunohistochemistry, and the correlation between ITGBL1 expression and the clinicopathological characteristics of the CRC patients was analyzed. Furthermore, the sh-ITGBL1 lentiviral system was used to construct ITGBL1 knockdown stablytransformed CRC cells, then the ITGBL1 protein expression was detected by Western blotting, and the effect of ITGBL1 knockdown on the proliferation capacity of CRC cells was assessed by CCK-8 assay and colony formation assay. Results: ITGBL1 was highly expressed in CRC tissues and cells. Its expression was positively correlated with the infiltration depth, lymph node metastasis and the clinical stage of CRC(all P<0.05). Compared with the control group, ITGBL1 expression was significantly decreased in ITGBL1 knockdown CRC cell lines(HCT116 and RKO), and the cell proliferation was significantly inhibited(all P<0.05). Conclusions: Highly expressed ITGBL1 might be associated with the occurrence and development of CRC. ITGBL1 knockdown exerts a significant inhibitory effect on the proliferation of CRC cells. Therefore, ITGBL1 might be a potential biomarker and novel target for diagnosis and treatment of CRC.更多还原