【摘要】 将来源于硬毛拴菌（Trametes trogii CICC 2689）的lcc1基因通过Di-CRISPR技术整合到酿酒酵母Y6中以实现漆酶的稳定表达，考察漆酶在酚类抑制剂及同步糖化发酵中的抗逆作用。结果显示：平板拮抗和液体摇瓶实验中，重组菌株Y6/Lac的生长状况、对数期生长速率及延滞期较对照菌株Y6均表现出相应优势；同时Y6/Lac对碱木质素的降解率提高41%，同步糖化发酵产乙醇效率提高14%。更多还原

【Abstract】 Lcc1 gene from Trametes trogii CICC 2689 was integrated into Saccharomyces cerevisiae Y6 by Di-CRISPR to achieve stable expression of laccase,and to investigate the stress resistance of laccase on phenolic inhibitors and simultaneous saccharification and fermentation. The results showed that the recombinant strains Y6/Lac in the medium containing inhibitors had shorter lag phase,higher exponential phase growth rate and final biomass than Y6. At the same time,the degradation rate of alkali lignin by Y6/Lac increased by41%,and the production efficiency of ethanol by simultaneous saccharification and fermentation increased by 14%.更多还原