【摘要】 为探讨不同种昆虫病原线虫（EPN）共生菌对根结线虫病的防治效果，开展了温室盆栽定量接种试验；为明确EPN共生菌的代谢产物对根结线虫卵孵化和二龄幼虫活性的影响，进行了室内生测试验。结果表明，Meloidogyne incognita（M. incognita）在中蔬4号番茄上接种剂量为1 000、2 000和5 000个卵·株^-1时，昆虫病原线虫共生菌Xb-IGA和Pl-IGA对M. incognita卵的繁殖均有较好的抑制效果，抑制效果为59.4%～73.9%。Meloidogyne hapla（M. hapla）在VFNT番茄上接种剂量为2 000个卵·株^-1时，昆虫病原线虫共生菌Pl-IGA对M. hapla卵的繁殖抑制效果最好，可达74.5%，与常规药剂阿维菌素（82.2%）差异不显著。4种EPN共生菌均能有效降低M. incognita和M. hapla侵染后的发病程度，对番茄根部生长有不同程度的促进作用。在生测试验7 d时，4种昆虫病原线虫共生菌代谢产物的2倍稀释液和5倍稀释液对M. incognita和M. hapla卵孵化抑制率为55.1%～94.8%，对M. incognita二龄幼虫校正死亡率为34.2%～47.8%，对M. hapla二龄幼虫校正死亡率为59.6%～79.9%。因此，昆虫病原线虫共生菌及其代谢产物对根结线虫是非常有潜力的生物杀线虫资源。更多还原

【Abstract】 In order to assess the control effects of symbiotic bacteria of different species entomopathogenic nematode（EPN） on Meloidogyne spp. as well as the metabolites of those symbiotic bacteria on egg hatching and larval activity of root-knot nematodes,we conducted a pot experiment with quantitative inoculation in greenhouse and a bioassay test in laboratory. The results showed that symbiotic bacteria of entomopathogenic nematode Xb-IGA and Pl-IGA exhibited good suppressive effect on the reproduction of M.incognita eggs, and the inhibition rate ranged 59.4% ～ 73.9% when M. incognita was inoculated with 1 000, 2 000 and 5 000 eggs·plant^-1 on Zhongshu 4 tomato. When M. hapla was inoculated with 2 000 eggs·plant^-1 on VFNT tomato, the inhibition rate of EPN symbiotic bacteria Pl-IGA reached the maximum value of 74.5%, which was not significantly different from the conventional control, i.e avermectin（82.2%）. Symbiotic bacteria of four species EPN effectively reduced the incidence of M. hapla and M. incognita infection, also promoted the root growth of tomato. After 7 days of bioassay, the 2× and 5× dilutions of metabolites by four species of symbiotic bacteria exhibited significant inhibition on the egg hatching of M. incognita and M. hapla. The corrected mortality of M. incognita larvae was 34.2% ～ 47.8%, and that of M. hapla was 59.6% ～ 79.9%. Therefore, symbiotic bacteria of entomopathogenic nematode and their metabolites showed great potential as biological nematicide resources for controlling root-knot nematode.更多还原