【摘要】 为探究药桑叶多糖(MLP)与传统降血糖药物胰岛素(INS)联合使用时的降血糖作用，本实验以壳聚糖(CTS)、药桑叶多糖为原料，三聚磷酸钠(TPP)为交联剂，INS为模型药物，通过离子交联法成功制备负载胰岛素的壳聚糖-药桑叶多糖(INS@CTS-MLP)纳米粒。结果表明，INS@CTS-MLP纳米粒的粒径为(464.61±2.28) nm, Zeta电位为(16.95±1.59) mV,包封率为(61.63±4.54)%,载药量为(12.22±1.23)%。体外释放试验表明在pH=1.2的酸性环境下，INS@CTS-MLP纳米粒能够有效减缓胰岛素的释放。体内降血糖活性试验表明给药4周时，INS@CTS-MLP纳米粒与MLP、INS@CTS纳米粒相比存在显著性差异(P<0.05)。其中MLP组血糖水平为模型组的60.07%(P<0.05),INS@CTS纳米粒组血糖水平为模型组的53.91%(P<0.05),INS@CTS-MLP纳米粒组血糖水平仅为模型组的40.23%(P<0.05),表明MLP和INS的联合使用对糖尿病型小鼠具有显著的协同降血糖作用。更多还原

【Abstract】 In order to investigate the hypoglycemic effect of Morus nigra L. leaves polysaccharide(MLP) combined with traditional hypoglycemic drug insulin(INS), chitosan(CTS) and MLP were used as raw materials, sodium tripolyphosphate( TPP) was used as crosslinking agent, and INS was used as model drug. Insulin-loaded chitosan-Morus nigra L. leaves polysaccharide(INS@CTS-MLP) nanoparticles were successfully prepared by ion crosslinking method. The results showed that the particle size of INS@CTS-MLP nanoparticles was(464.61±2.28) nm, the Zeta potential was(16.95±1.59) mV, the encapsulation efficiency was(61.63±4.54)%, and the drug loading was(12.22±1.23)%. In vitro release test showed that INS@CTS-MLP nanoparticles could effectively slow down the release of insulin in an acidic environment with pH=1.2. In vivo hypoglycemic activity test showed that INS@CTS-MLP nanoparticles had significant difference compared with MLP and INS@CTS nanoparticles at 4 weeks of administration(P<0.05). The blood glucose level of the MLP group was 60.07% of that of the model group(P<0.05), the blood glucose level of the INS@CTS nanoparticle group was 53.91% of that of the model group(P<0.05), and the blood glucose level of the INS@CTS-MLP nanoparticle group was only 40.23% of that of the model group(P<0.05), which proved that the combined use of MLP and INS had a significant synergistic hypoglycemic effect on diabetic mice.更多还原