【摘要】 该文以橙皮为原料制备的碳量子点（CDs）与铕离子(Eu³⁺)构建双发射比率型荧光探针(Eu³⁺-CDs)并用于抗生素盐酸四环素的检测。在370 nm激发波长下，Eu³⁺-CDs荧光探针在425 nm处出现较强的蓝色荧光峰，在617 nm处出现较弱的红色荧光峰。随着盐酸四环素浓度的逐渐增大，Eu³⁺与盐酸四环素螯合形成的配合物增多，在425 nm处的CDs荧光(I_F425)逐渐减弱，而617 nm处铕配合物的荧光(I_F617)逐渐增强，可基于荧光强度比I_F617/I_F425的变化对盐酸四环素进行测定。考察了Eu³⁺的浓度、pH值和反应时间对探针荧光强度的影响。在最佳条件下，该双发射比率型荧光探针对盐酸四环素具有特异性识别能力，荧光强度比I_F617/I_F425与盐酸四环素浓度在10～100μmol/L范围内存在良好的线性关系，相关系数r²=0.992 6，检出限为5.09μmol/L。该方法成功用于猪肉中盐酸四环素含量的检测，加标回收率为102%～110%，相对标准偏差为0.20%～2.4%，具有较好的实用性。更多还原

【Abstract】 Carbon quantum dots（CDs） prepared from orange peel were employed to construct a dualemission ratiometric fluorescent probe(Eu³⁺-CDs) with europium ions(Eu³⁺) for the detection of the antibiotic tetracycline hydrochloride. The Eu³⁺-CDs fluorescent probe exhibits two distinct fluorescence peaks at 370 nm excitation:a robust blue peak at 425 nm and a comparatively moderate red peak at 617 nm. With the concentration of tetracycline hydrochloride increased,Eu³⁺ chelated with tetracycline hydrochloride to form more complexes. As a result,the fluorescence of CDs at 425 nm（IF425） steadily dropped while that of europium complexes at 617 nm(I_F617) increased. This variation in fluorescence intensity ratio of I_F617/I_F425 allowed for the measurement of the tetracycline hydrochloride content. The effects of Eu³⁺ concentration,pH value and reaction time on the fluorescence intensity were investigated. Under the optimal conditions,the dual-emission ratiometric fluorescent probe had a specific recognition ability for tetracycline hydrochloride. A good linear relationship between the fluorescence intensity ratio(I_F617/I_F425 and the concentration of tetracycline hydrochloride（10-100 μmol/L） was obtained. The linear equation was I_F617/I_F425= 0. 015 61c（μmol/L） + 0. 050 81,and the linear correlation coefficient was r² = 0. 992 6 with the detection limit of 5. 09 μmol/L. The method was successfully utilized to detect the content of tetracycline hydrochloride in pork with the spiked recoveries of 102% to 110% and the relative standard deviations of 0. 20%-2. 4%.更多还原