【摘要】 目的 探讨过敏原对健康人（healthy control,HC）和过敏性鼻炎（allergic rhinitis,AR）患者外周血CD4~+Th1细胞表达IL-18、IL-18BPa和IL-18Rα蛋白水平及mRNA水平的影响。方法 收集皮肤点刺试验阴性的鼻炎（AR-）、皮肤点刺试验阳性的鼻炎（AR+）患者和HC的外周血标本。流式细胞术检测过敏原对CD4~+Th1细胞表达IL-18、IL-18BPa和IL-18Rα蛋白水平的影响；qPCR检测过敏原对CD4~+T细胞表达IL-18、IL-18BPa和IL-18Rα mRNA水平的影响。结果 与HC相比，AR-和AR+患者外周血Th1细胞中IL-18蛋白的表达增加，IL-18BPa蛋白的表达减少；AR+患者外周血Th1细胞中IL-18Rα蛋白的表达增加；过敏原可增强HC Th1细胞IL-18Rα蛋白的表达，并诱导AR+患者和HC CD4~+T细胞IL-18、IL-18BPa和IL-18Rα mRNA的表达。结论 过敏原极可能通过促进外周血CD4~+Th1细胞表达IL-18和IL-18Rα进而参与AR发病。更多还原

【Abstract】 Objective To investigate the effects of allergens on the expressions of IL-18, IL-18BPa and IL-18Rα protein in peripheral blood CD4~+ Th1 cells of healthy control subjects(HC) and patients with allergic rhinitis(AR), and on the expressions of IL-18, IL-18BPa and IL-18Rα mRNA in the peripheral blood CD4~+ T cells.Methods Blood samples were collected from patients with rhinitis for negative skin prick test(AR-), rhinitis for positive skin prick test(AR+) and HC. Flow cytometry was used to evaluate the effects of allergens on the expressions of IL-18, IL-18BPa and IL-18Rα protein in CD4~+ Th1 cells. The expressions of IL-18, IL-18BPa and IL-18Rα mRNA in CD4~+ T cells were determined by qPCR. Results Compared with HC, increased IL-18 while decreased IL-18BPa expressions in Th1 cells of AR-and AR+ patients were observed, increased IL-18Rα expression in Th1 cells of AR+ patients was also found. Additionally, allergens induced elevated expression of IL-18Rα protein in Th1 cells of HC, and induced elevated mRNA expressions of IL-18, IL-18BPa and IL-18Rα in isolated blood CD4~+ T cells of AR+ patients and HC. Conclusion Allergens may be involved in the pathogenesis of AR by inducing the expressions of IL-18 and IL-18Rα in blood CD4~+ Th1 cells.更多还原