【摘要】 目的 研究雷公藤甲素对骨肉瘤细胞SaOS-2增殖、侵袭和凋亡的影响和机制。方法 骨肉瘤细胞SaOS-2分成Control组、Triptolide组(40、80、160 nmol·L^-1雷公藤甲素)、阳性对照组(15 mg·L^-1 5-氟尿嘧啶)、Triptolide+si-NC组(转染siRNA control, 160 nmol·L^-1雷公藤甲素)、Triptolide+si-BE503655组(转染BE503655 siRNA,160 nmol·L^-1雷公藤甲素),以CCK-8实验分析细胞变化，用流式细胞术分析细胞凋亡变化，以Transwell小室分析细胞迁移和侵袭变化，Western blot分析神经性钙黏附素（neural cadherin, N-cadherin）、上皮性钙黏附素（epithelical cadherin, E-cadherin）、Bcl-2相关X蛋白（Bcl-2 associated X protein, Bax）、Bcl-2相关X蛋白（Bcl-2 associated X protein, Bax）、基质金属蛋白酶-2（matrix metalloprotease 2,MMP-2）蛋白表达变化，qRT-PCR方法分析BE503655表达。结果 与Control组比较，Triptolide组(40、80、160 nmol·L^-1雷公藤甲素)、阳性对照组骨肉瘤细胞存活率降低，细胞凋亡率升高，侵袭数目和迁移数目减少，细胞中Bax、E-cadherin蛋白表达水平增加，Bcl-2、MMP-2、N-cadherin蛋白表达水平降低，BE503655表达水平升高。与Triptolide+si-NC组比较，Triptolide+si-BE503655组骨肉瘤细胞存活率升高，细胞凋亡率降低，迁移和侵袭数目均增加，MMP-2、N-cadherin、Bcl-2蛋白表达水平均升高，E-cadherin、Bax蛋白表达水平均降低，BE503655水平降低。结论 雷公藤甲素通过上调BE503655发挥抑制骨肉瘤细胞SaOS-2增殖、迁移、侵袭、EMT和促凋亡作用。更多还原

【Abstract】 Objective To study the effect of triptolide on the proliferation, invasion and apoptosis of osteosarcoma cell SaOS-2.Methods SaOS-2 osteosarcoma cells were divided into Control group, Triptolide group(40,80,160 nmol·L^-1 triptolide),positive control group(15 mg·L^-1 5-fluorouracil),Triptolide+si-NC group(transfected siRNA control, 160 nmol·L^-1 triptolide),Triptolide+si-BE503655 group(transfected with BE503655 siRNA,160 nmol·L^-1 triptolide),CCK-8 experiment was used to analyze cell changes, flow cytometry was used to analyze cell apoptosis, and Transwell chamber was used to analyze cell migration and invasion changes, Western blot was used to analyze N-cadherin, E-cadherin, Bax, Bcl-2,MMP-2 protein expression changes, qRT-PCR method was used to analyze the expression of BE503655.Results Compared with the control group, the survival rate of osteosarcoma cells in the Triptolide group(40,80,160 nmol·L^-1 triptolide) and positive control group were decreased, the apoptosis rate was increased, and the number of invasion and migration were decreased.Bax, E-cadherin protein expression level were increased, Bcl-2,MMP-2,N-cadherin protein expression level was decreased, and BE503655 expression level was increased.Compared with the Triptolide+si-NC group, the osteosarcoma cell survival rate in the Triptolide+si-BE503655 group was increased, the apoptosis rate was decreased, the number of migration and invasion were increased, and the protein expression levels of MMP-2,N-cadherin and Bcl-2 were increased, and the expression levels of E-cadherin and Bax protein were decreased, the BE503655 level was decreased.Conclusion Triptolide exerts the effects of inhibiting proliferation, migration, invasion, EMT and promoting apoptosis of osteosarcoma cell SaOS-2 by up-regulating BE503655.更多还原