【摘要】 探究高产胞外多糖（exopolysaccharides,EPS）菌株的渣土改良性能，为解决客土资源缺乏的弃渣改良问题提供新的修复措施及菌种资源。从渣土中原位分离筛选出3株高产EPS土壤细菌，并将3株菌株作为供试复合菌剂，以渣土配比、颗粒级配、菌剂浓度和菌剂用量为正交试验因素进行干旱胁迫条件下的盆栽试验，采用主成分分析优化种植基质配比，遴选出土壤资源合理化利用的的最优修复基质配比。结果表明：3株菌株分别为耐寒短杆菌（Peribacillus frigoritolerans）、蜡状芽孢杆菌（Bacillus cereus）和贝莱斯芽孢杆菌（Bacillus velezensis）。渣土改良基质的最优配比为营养土∶渣土=7∶3，渣土颗粒级配5 mm，菌剂浓度0.6(OD₆₀₀)，菌剂用量150 mL·kg^-1。施用高产EPS菌剂是实现干旱区渣土改良的有效措施，但其应用效率受土壤质地及结构特征的影响。高产EPS菌剂可有效改善渣土结构、理化性质及酶活性，并帮助植物抵御干旱胁迫影响，在弃渣土改良方面具有良好的应用潜力。更多还原

【Abstract】 This study explores the soil improvement potential of high-yield exopolysaccharide （EPS） strains for waste soil,providing new remediation strategies and microbial resources to address the shortage of topsoil.Three high-yield EPS-producing soil bacteria were isolated in situ from waste soil:Peribacillus frigoritolerans,Bacillus cereus and Bacillus velezensis.These strains were used as composite inoculants in pot experiments under drought stress,with soil-to-waste ratio,particle size distribution,inoculant concentration,and inoculant dosage as orthogonal test factors.Principal component analysis was employed to optimize the substrate formulation,resulting in the optimal remediation ratio:nutrient soil-towaste soil=7∶3,particle size distribution=5 mm,inoculant concentration=0.6 (OD₆₀₀),and inoculant dosage=150 mL·kg^-1.The application of high-yield EPS inoculants was found to be an effective strategy for improving waste soil in arid regions.However,its efficiency is influenced by soil texture and structural characteristics.EPS-producing inoculants significantly improved the structure,physicochemical properties,and enzyme activity of waste soil,helping plants resist drought stress.These findings highlight the strong potential of EPS inoculants for waste soil remediation.更多还原