【摘要】 基于绿色分析化学理念，设计了液液微萃取-高效液相色谱（HPLC）法测定水中磺胺类药物的实验并应用于本科实践教学。实验中采用较少的萃取溶剂对样品进行前处理，色谱分离时选用乙醇作为绿色有机流动相，通过优化萃取和分离条件，建立了一种液液微萃取-高效液相色谱联用分析水样中磺胺类药物（磺胺胍（SG）、磺胺嘧啶（SD）、磺胺间二甲氧嘧啶（SDM）、磺胺甲噁唑（SMZ）和酞磺胺噻唑（PST））及其常用配伍药甲氧苄啶（TMP）的新方法。其结果表明测定各药物的线性范围分别为10～1 000μg/L(SG、SD、SDM及SMZ)、100～1 000μg/L(TMP)和50～1 000μg/L(PST)，检测限在50～100 ng/L(S/N=3)之间。对湖水样品进行测定，加样回收率在93.6%～105.2%之间。该实验立足于分析化学前沿且难易适中，可以作为化学、药学和环境等专业高年级本科生的研究性实验，有利于学生提高创新能力、低碳意识及综合素质。更多还原

【Abstract】 With the concept of green analytical chemistry, an experiment on the determination of sulfonamide drugs in water using liquid-liquid microextraction combined with high-performance liquid chromatography(HPLC) is designed for undergraduate experimental teaching. With a small amount of organic solvents in liquid-liquid extraction and ethanol as the green organic mobile phase for chromatographic separation, the extraction and separation conditions are optimized, and a new method of liquid-liquid microextraction combined with HPLC has been developed for determining sulfonamide drugs(sulfaguanidine(SG), sulfamethoxazole(SD), sulfamethoxazole(SDM), sulfamethoxazole(SMZ) and sulfamethoxazole(PST)) and trimethoprim(TMP, a commonly used compatible drug for sulfonamide drugs). The results indicate that the linear measurement ranges are 10～1 000 μg/L for SG, SD,SDM and SMZ, and 100～1 000 μg/L for TMP and 50～1 000 μg/L for PST, respectively. The detection limits are in the ranges of50～100 ng/L(S/N=3). The recovery rates of the lake water sample are between 93.6% and 105.2%. This experiment is based on the forefront of analytical chemistry and is moderately difficult. It is suitable as a researching experiment for senior undergraduate students majoring in chemistry, pharmacy and environment, which is beneficial for students to improve their innovation ability, low-carbon awareness and comprehensive quality.更多还原