【摘要】 合成了一种多吡啶单核镍金属配合物（Ni（acac）₂（o-NPIP）]（CH₃OH）₃），利用光谱和单晶衍射对镍配合物进行了表征，采用紫外吸收光谱、荧光猝灭光谱揭示镍配合物与DNA的结合方式，利用荧光光谱法研究镍配合物与人血清白蛋白（HSA）的猝灭机制、结合常数及作用方式。结果表明：镍配合物是六配位单核不对称的八面体结构，镍配合物以插入方式与DNA进行结合，结合常数K_b=1.17×10⁴ L·mol^-1。随着镍配合物加入，溴化乙啶（EB）-DNA的荧光强度呈现下降趋势，意味着镍配合物与EB之间进行了竞争，取代EB与DNA进行结合，表观键合常数K_app=8.03×10⁵ L·mol^-1，镍配合物与DNA的键合能力中等。镍配合物以静态猝灭机理导致HSA的荧光猝灭，且镍配合物与HSA的结合常数K_b均为10⁵数量级，表明有较强的结合能力。镍配合物与HSA的主要结合力为疏水作用力。同步荧光研究发现，色氨酸残基是镍配合物和HSA的结合点。因此，合成的新型多吡啶镍配合物与DNA的结合能力中等，且能够以较强的结合能力与HSA结合，具有较好的生物活性。更多还原

【Abstract】 A poly pyridine mononuclear nickel metal complex（Ni（acac）₂（o-NPIP）]（CH₃OH）₃） was synthesized,the nickel complexes were characterized by spectrum and single crystal diffraction.The binding mode of nickel complex and DNA was revealed by ultraviolet absorption spectrum and fluorescence quenching spectrum.The quenching mechanism,binding constant and action mode of nickel complex and HSA were studied by fluorescence spectroscopy.The results showed that the nickel complexes were six-coordinated mononuclear asymmetric octahedral structures.The nickel complex bonded to DNA by insertion,and the binding constant K_b=1.17×10⁴ L·mol^-1.With the addition of nickel complex,the fluore scence intensity of EB-DNA showed a decreasing trend,indicating that the nickel complex competed with EB to replace EB in binding DNA.The apparent bonding constant K_app=8.03×10⁵ L·mol^-1,the nickel complex had moderate bonding DNA capacity.The fluorescence quenching of HSA was caused by the static quenching mechanism of nickel complex,and the binding constant K_b of nickel complex and HSA was 10⁵ orders of magnitude,indicating a strong binding ability.The main binding force between nickel complexes and HSA was the hydrophobic force.Synchronous fluorescence study showed that tryptophan residue was the binding point of nickel complex and HSA.So,the new polypyridine nickel complex synthesized had moderate binding ability to DNA,and could bind to HSA with strong binding ability,and had good biological activity.更多还原