【摘要】 目的 探讨前列腺癌组织中微小RNA-647(miR-647)、微小RNA-122(miR-122)、组蛋白赖氨酸甲基转移酶2D(KMT2D)与其临床病理特征及预后的相关性。方法 选取100例前列腺癌患者作为研究对象，均于术中采集肿瘤组织及癌旁正常组织标本送检，统计肿瘤组织及癌旁正常组织内miR-647、miR-122、KMT2D表达水平差异；分析miR-647、miR-122、KMT2D表达与肿瘤分期、肿瘤直径、淋巴结转移、微血管侵犯等的关系；并随访2年，比较复发转移与未复发转移患者miR-647、miR-122、KMT2D表达间差异。结果 肿瘤组织内miR-647表达水平为(0.62±0.13),低于癌旁正常组织的(1.02±0.19),miR-122、KMT2D表达水平为(6.45±1.24)、(1.45±0.23),低于对照组的(1.23±0.22)、(0.89±0.12)(P<0.05);肿瘤分期Ⅲ期、肿瘤直径≥3 cm、有淋巴结转移及有微血管侵犯患者miR-647表达水平低于肿瘤分期Ⅰ～Ⅱ期、肿瘤直径<3 cm、无淋巴结转移及无微血管侵犯患者，miR-122、KMT2D表达水平高于肿瘤分期Ⅰ～Ⅱ期、肿瘤直径<3 cm、无淋巴结转移及无微血管侵犯患者(P<0.05);100例患者术后随访2年，共出现24例复发转移，复发转移率为24.00%(24/100);复发转移患者miR-647表达水平为(0.51±0.11),低于未复发转移患者的(0.73±0.15),miR-122、KMT2D表达水平为(8.42±1.39)、(1.89±0.32),高于未复发转移患者的(4.68±1.02)、(1.12±0.15)(P<0.05)。结论 前列腺癌组织内miR-647、miR-122、KMT2D存在不同程度表达，均与肿瘤分期、肿瘤直径及淋巴结转移等关系密切，且可明显影响患者预后，还需临床高度重视。更多还原

【Abstract】 Objective To investigate the correlation between minimal RNA-647(miR-647),minimal RNA-122(miR-122),histone lysine methyltransferase 2D(KMT2D),clinical pathological features, and prognosis in prostate cancer tissue.Methods 100 patients with prostate cancer were selected as the research subjects, and tumor tissue and adjacent normal tissue samples were collected during surgery for examination.The expression levels of miR-647,miR-122,and KMT2D in tumor tissue and adjacent normal tissue were statistically analyzed; Analyzed the relationship between miR-647,miR-122,KMT2D expression and tumor staging, tumor diameter, lymph node metastasis, microvascular invasion, etc; And followed up for 2 years to compare the differences in miR-647,miR-122,and KMT2D expression between patients with recurrence and metastasis and those without recurrence and metastasis.Results The expression level of miR-647 in tumor tissue was(0.62±0.13),lower than that in normal tissues adjacent to cancer(1.02±0.19).The expression levels of miR-122 and KMT2D were(6.45±1.24) and(1.45±0.23),lower than those in the control group(1.23±0.22) and(0.89±0.12)(P<0.05);The expression levels of miR-647 in patients with tumor stage III,tumor diameter ≥ 3cm, lymph node metastasis, and microvascular invasion were lower than those in patients with tumor stage I-II,tumor diameter<3cm, no lymph node metastasis, and no microvascular invasion.The expression levels of miR-122 and KMT2D were higher than those in patients with tumor stage I-II,tumor diameter<3 cm, no lymph node metastasis, and no microvascular invasion(P<0.05);100 patients were followed up for 2 years after surgery, with a total of 24 cases experiencing recurrence and metastasis, with a recurrence and metastasis rate of 24.00%(24/100);The expression level of miR-647 in patients with recurrence and metastasis was(0.51±0.11),lower than that in patients without recurrence and metastasis(0.73±0.15).The expression levels of miR-122 and KMT2D were(8.42±1.39) and(1.89±0.32),higher than those in patients without recurrence and metastasis(4.68±1.02) and(1.12±0.15)(P<0.05).Conclusion miR-647,miR-122,and KMT2D are expressed to varying degrees in prostate cancer tissue, which are closely related to tumor staging, tumor diameter, and lymph node metastasis, and can significantly affect patient prognosis.Clinical attention should be paid to these factors.更多还原