【摘要】 目的：探讨p16蛋白与HPV-DNA、TCT联合应用在宫颈癌病变筛查的价值研究。方法：回顾性分析2023年02月至2023年12月在广州市某地区妇幼保健院就诊疑似患者3952例，并行p16蛋白检测与HPV-DNA筛查、液基薄层细胞（TCT）以及宫颈癌病理切片（HE）鉴定，分析、比较不同年龄段组女性p16蛋白免疫组化、HPV-DNA分型以及TCT检测结果相关性。结果：p16蛋白检测阳性率随着HPV-DNA筛查增高有递增趋势（差异具有统计学意义，P<0.05）。p16蛋白检测随着TCT诊断结果分级NILM与ASC-US、ASC-H、LSIL、HSIL、SCC组别的升高有递增趋势（差异具有统计学意义，P<0.05）。p16蛋白检测随着宫颈癌病理切片HE染色分级增高有递增趋势（差异具有统计学意义，P<0.05）。p16蛋白检测阳性率与患者年龄关系密切；在3952例妇女中HPV-DNA筛查阳性患者1836例，阳性率约46.46%;TCT检测结果阳性有1528名，阳性率约38.66%；按年龄分组结果：发病年龄30岁以后有上升趋势，41-50岁是发病高峰年龄段，60岁以后发病率下降，年龄呈抛物线向上及下趋势；宫颈癌发病还有呈年轻化趋势。结论：p16蛋白与HPV-DNA、TCT联合应用提高了宫颈癌及其癌前病变筛查的准确度。更多还原

【Abstract】 Objective: To investigate the effect p16 protein combined with HPV-DNA and TCT in screening cervical cancer lesions. Methods: A retrospective analysis was conducted on 3952 suspected patients who visited a women’s and children’s health hospital in a certain area of Guangzhou from February 2023 to December 2023. Parallel p16 protein detection, HPV-DNA screening,Thin-prep cytologic test(TCT), and cervical cancer histopathological section(HE) identification were performed. The correlation between p16 protein immunohistochemistry, HPV-DNA typing, and TCT test results in different age groups of women was analyzed and compared. Results: The positive rate of p16 protein detection tended to increase with the increase in HPV-DNA screening(the difference was statistically significant, P<0.05). p16 protein detection tended to increase with the grading of TCT diagnosis results from NILM to ASC-US, ASC-H, LSIL, HSIL, and SCC groups(the difference was statistically significant, P<0.05). p16 protein detection tended to increase with the grading of cervical cancer histopathological sections by HE staining(the difference was statistically significant, P<0.05). The positive rate of p16 protein detection was closely related to patient age; among the 3952 women, 1836 were HPV-DNA screening positive, with a positive rate of approximately 46.46%; 1528 were TCT positive, with a positive rate of approximately 38.66%;grouped by age, the onset age tended to increase after 30 years old, peaked at 41-50 years old, and decreased after 60 years old, showed an upward and downward parabolic trend with age; the onset of cervical cancer also showed a trend of younger age. Conclusion: The combination of p16 protein, HPV-DNA, and TCT improved the accuracy of cervical cancer and precancerous lesion screening.更多还原