【摘要】 目的：探讨下调DEAD-box解旋酶1(DDX1)基因对宫颈鳞癌Si Ha细胞增殖、凋亡、转移及侵袭能力的影响。方法：si RNA转染构建DDX1低表达Si Ha细胞系、和阴性对照组，用RT-qPCR和Westem Blot检测DDX1的m RNA和蛋白表达水平。应用CCK8法、流式细胞技术、Transwell实验分别检测DDX1低表达对Si Ha细胞增殖、凋亡、转移和侵袭能力的影响。结果：（1）m RNA水平检测显示：下调DDX1,Si-DDX1组中DDX1表达均低于Si-NC组，差异有统计学意义（P<0.05）。蛋白水平检测显示，Si-DDX1组中DDX1表达低于Si-NC组，差异有统计学意义（P<0.05）。（2）下调DDX1后，Si-DDX1组细胞的增殖活性较Si-NC组升高，Si-DDX1组的凋亡率较Si-NC组降低，差异有统计学意义（P<0.05）。（3）下调DDX1后，Si-DDX1组细胞转移率和侵袭率均较Si-NC组增高，差异有统计学意义（P<0.05）。结论：DDX1的表达受到抑制后，宫颈鳞癌Si Ha细胞的增殖能力增强、凋亡能力减弱，转移和侵袭能力增强。更多还原

【Abstract】 Objective: To investigate the impact of downregulating DDX1 gene on growth, apoptosis, metastasis, and invasion of Si Ha cells in cervical cancer. Methods: A DDX1-low-expression Si Ha cell line was established via si RNA transfection, alongside a negative control group. The m RNA and protein expression levels of DDX1 were assessed using RT-qPCR and Western Blot analysis, respectively. Additionally, CCK8 assay, flow cytometry analysis, and Transwell assay were conducted to evaluate the effect of decreased DDX1expression on proliferation, apoptosis, metastasis, and invasion of Si Ha cells. Results:(1) Analysis of m RNA levels revealed significantly lower expression of DDX1 in the Si-DDX1 group compared to the Si-NC group(P<0.05). Similarly, analysis at the protein level demonstrated a statistically significant decrease in DDX1 expression in the Si-DDX1 group compared to the Si-NC group(P<0.05).(2)Down-regulation of DDX1 resulted in increased cellular proliferation activity in the Si-DDX1 group compared to the Si-NC group(P<0.05), while exhibiting a lower rate of apoptosis(P<0.05).(3) Decreased expression of DDX1 led to higher rates of metastasis and invasion in the Si-DDX1 group compared to the Si-NC group with statistical significance(P<0.05). Conclusions: Inhibition or reduction of DDX1expression enhances cellular proliferation ability while weakening apoptotic capability; additionally promoting metastatic potential and invasive abilities within Si Ha cells.更多还原