【摘要】 目的：比较山药皮多酚(Yam peel polyphenols, YPP)提取工艺，并评价其抗氧化活性。方法：通过单因素试验和正交试验，优化超声辅助酶解法和微波辅助酶解法提取YPP的最佳工艺，并比较2种方法得到YPP提取物的体外抗氧化能力。结果：超声辅助酶解法优于微波辅助酶解法，其最佳提取条件为料液比1:15 g/mL、酶解温度60℃、超声时间30 min、超声功率300W、酶浓度20mg/g、乙醇体积分数75%、pH5,YPP提取率为2.3865mg/g，是微波辅助酶解法提取率的1.33倍，是传统乙醇静置提取法提取率的2.34倍。对提取后的残渣进行扫描电镜观察，结果显示与传统静置提取法和微波辅助酶解法相比，超声辅助酶解法使残渣结构破坏更显著，更有利于YPP溶出。体外抗氧化研究表明，超声辅助酶解法提取得到的YPP在1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl, DPPH)自由基、2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonicacid),ABTS)阳离子自由基、超氧阴离子自由基和羟自由基清除率上均明显高于微波辅助酶解法。结论：超声辅助酶解法提取YPP提取率较高，且提取物具有较强的抗氧化活性，可作为YPP的较佳提取方法。更多还原

【Abstract】 Objective: This study compared the extraction process of yam peel polyphenols(YPP),and evaluated the in vitro antioxidant activity. Methods: Ultrasonic-assisted enzymatic hydrolysis and microwave-assisted enzymatic hydrolysis extraction process of YPP were optimized by single factor tests and orthogonal tests, the antioxidant capacity of YPP extracts obtained by two methods was compared.Results: The results showed that ultrasound-assisted enzymatic hydrolysis was superior to microwaveassisted enzymatic hydrolysis, optimal extraction conditions of ultrasound-assisted enzymatic hydrolysis were as follows: solid-liquid ratio 1:15 g/mL, enzymatic temperature 60 ℃, ultrasonic time 30 min,ultrasonic power 300 W, enzyme hydrolysis concentration 20 mg/g, ethanol concentration 75%, pH5, The YPP extraction yield was 2.3865 mg/g, which was 1.33 times higher than that of the microwave-assisted enzymatic hydrolysis method and 2.34 times higher than the traditional ethanol static extraction method.Scanning electron microscopy(SEM) of the extracted residues showed that the ultrasonic-assisted enzymatic method caused more significant structural damage compared to the traditional static extraction and microwave-assisted enzymatic hydrolysis methods, facilitating better YPP release. In vitro antioxidant studies have shown, the removal rates of 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and 2,2’-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS) radical cation, hydroxyl and superoxide anion radical of YPP extracted by ultrasound-assisted enzymatic hydrolysis were significantly higher than those obtained by microwave-assisted enzymatic hydrolysis method. Conclusion: The ultrasonic-assisted enzymatic hydrolysis method yielded a higher extraction rate of YPP, and the extracts exhibited strong antioxidant activity, making it a superior extraction method for YPP.更多还原