【摘要】 建立一种快速的高效液相色谱法用于分析检测调制乳粉中维生素K₂的含量。取调制乳粉样品，加入脂肪酶充分酶解，以一定比例的无水乙醇/正丁醇（10:10，V:V）提取，异丙醇充分稀释，定容，0.2 μm滤膜过滤过滤。采用高效液相色谱法进行测定，色谱柱为Welch Ultimate^? AQ-C₁₈（4.6 mm×150 mm，5 μm），流动相为100%甲醇，流速1.0 mL/min，进样体积20 μL，柱温35 ℃，检测波长254 nm，以外标法定量。维生素K₂在0.1～5.0 μg/mL浓度范围内呈良好的线性关系，标准曲线的相关系数R²为1.000。加标回收率在103.00%～106.68%之间，精密度试验中维生素K₂含量的相对标准偏差为1.7%，检出限为0.0508 μg/mL。此方法操作简单、准确性好、精密度高，适用于调制乳粉中维生素K₂含量测定。更多还原

【Abstract】 A rapid high performance liquid chromatography （HPLC） method for analyzing the content of vitamin K₂ in milk powder was established.Fortified milk powder samples were enzymatically hydrolyzed with lipase,extracted with a mixture of anhydrous ethanol/normal butanol （10:10,V:V） in a certain proportion,fully diluted with isopropanol,and filtered through a 0.2 μm membrane filter.Using HPLC for analysis,the chromatographic column used was the Welch Ultimate^? AQ-C₁₈ （4.6 mm×150 mm,5 μm）,the mobile phase was 100% methanol,the flow rate was 1.0 mL/min,and the injection was 20 μL,the column temperature was 35 ℃,and a detection wavelength of 254 nm.The standard curve of vitamin K₂ was linear in the range of 0.1–5.0 μg/mL （R²=1.000）.The standard recovery was 103.00%–106.68%,the relative standard deviation （RSD） of vitamin K₂ in precision experiment was 1.7%,the limit of detection was 0.0508 μg/mL.The method is simple,accurate and accurate,making it suitable for the determining the content of vitamin K₂ in milk powder.更多还原