【摘要】 文章探讨超声波辅助提取芡叶总黄酮的工艺，以芡叶总黄酮得率为评价指标，乙醇体积分数、料液比、超声时间和超声温度为影响因素，采用单因素实验和Box-Behnken(BBD)实验设计优化芡叶总黄酮的提取工艺，并测定其体外抗油脂氧化能力。结果表明：最佳工艺条件为乙醇体积分数68%、料液比1:19 g/mL、超声时间30 min、超声温度49℃，芡叶总黄酮得率为(1.69±0.12)%。芡叶总黄酮能够明显减缓油脂过氧化值(Peroxidation value, POV)值的升高趋势，且具有一定的浓度依赖性。在高温诱导后的第16天，加入0.05 mg/g芡叶总黄酮的动物油脂，其POV值为(15.2±1.16)mmol/kg，仅为空白对照组的33.9%；同样实验条件下的植物油脂，其POV值为(32.1±2.15)mmol/kg，仅为空白对照组的38.6%，二者数值上均接近于加入量为0.03 mg/g丁基羟基茴香醚(Butyl hydroxyanisole, BHA)的抗氧化效果。该实验模型设计达到要求，工艺条件可行、可靠，芡叶总黄酮具有一定的体外抗油脂氧化能力，为当地芡叶资源的充分开发利用提供理论依据。更多还原

【Abstract】 The extraction process of total flavonoids from leaves of Euryale ferox by ultrasonic-assisted was explored, single factor test and Box-Behnken(BBD) experimental design were used to optimize the extraction process with the yield of total flavonoids from leaves of Euryale ferox as the response, and the volume fraction of ethanol, solid/liquid ratio, ultrasonic time and ultrasonic temperature were used as independent variables, and the anti-lipid oxidation ability of flavonoids from leaves of Euryale ferox in vitro was analyzed. The results showed that, the optimal extraction process was as follows: 68% for volume fraction of ethanol, 1:19 g/mL for solid/liquid ratio, 30 min for ultrasonic time and 49 ℃ for ultrasonic temperature, the yield of total flavonoids was(1.69±0.12)%. Total flavonoids from leaves of Euryale ferox could significantly slow the increasing trend of oil peroxidation value(POV), with a certain amount of concentration-dependent. POV value of animal fat added with 0.05 mg/g total flavonoids from leaves of Euryale ferox was(15.2±1.16)mmol/kg, which was only 33.9% of the blank control group on the 16th day after high temperature induction, and the POV value of vegetable oil under the same experimental conditions was(32.1±2.15)mmol/kg, which was only 38.6% of the blank control group, both values were close to the amount of butyl hydroxyanisole(BHA) added 0.03 mg/g. The process conditions were feasible and reliable, the total flavonoids from leaves of Euryale ferox had a certain anti-lipid oxidation ability and provided a theoretical basis for the full development and utilization of local leaves of Euryale ferox resources.更多还原