【摘要】 褐腐病是采后桃果实最主要的侵染性病害之一，而我国桃褐腐病主要是由链核盘菌属引起，会严重降低果实品质，造成大量损失。为解析桃果实病原菌胁迫响应机制，该文采用分光光度法测定抗病相关酶活性并基于转录组学解析桃果实响应果生链核盘菌的分子机制。结果发现，桃果实的过氧化物酶、苯丙氨酸解氨酶和β-1,3-葡聚糖酶在侵染的不同时期启动了响应反应，参与了桃果实抵御果生链核盘菌侵染过程。基因本体（gene ontology,GO）和京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes,KEGG）富集分析表明，桃果实对果生链核盘菌的侵染会产生复杂的防御反应。更多还原

【Abstract】 Brown rot is one of the main infectious diseases in postharvest peaches. In China,brown rot of peaches is mainly caused by Monilinia fructicola,which can seriously reduce fruit quality and cause significant losses. To analyze the response mechanism of pathogen stress of peaches,spectrophotometry was used to measure the activity of disease-resistant enzymes and explore the molecular mechanism of peach response to Monilinia fructicola based on transcriptomics. It was found that the peroxidase(POD),phenylalanine ammonia lyase(PAL),and β-1,3-glucanase(GLU)in peach initiated response reactions at different stages of infection,participating in the resistance of peach to the infection of Monilinia fructicola. Moreover,Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis indicated that peaches could exhibit complex defense responses against the infection of Monilinia fructicola.更多还原